Epitopes described in "Characterization of the specific CD4+ T cell response against the F protein during chronic hepatitis C virus infection."

Article Authors:De-Yong Gao; Gen-Di Jin; Bi-Lian Yao; Dong-Hua Zhang; Lei-Lei Gu; Zhi-Meng Lu; Qiming Gong; Yu-Chun Lone; Qiang Deng; Xin-Xin Zhang
Article Title:Characterization of the specific CD4+ T cell response against the F protein during chronic hepatitis C virus infection.
Reference Detail
Reference ID:1021355
Abstract:BACKGROUND: The hepatitis C virus (HCV) Alternate Reading Frame Protein (ARFP or F protein) presents a double-frame shift product of the HCV core gene. We and others have previously reported that the specific antibodies against the F protein could be raised in the sera of HCV chronically infected patients. However, the specific CD4(+) T cell responses against the F protein during HCV infection and the pathological implications remained unclear. In the current study, we screened the MHC class II-presenting epitopes of the F protein through HLA-transgenic mouse models and eventually validated the specific CD4(+) T cell responses in HCV chronically infected patients. METHODOLOGY: DNA vaccination in HLA-DR1 and-DP4 transgenic mouse models, proliferation assay to test the F protein specific T cell response, genotyping of Chronic HCV patients and testing the F-peptide stimulated T cell response in the peripheral blood mononuclear cell (PBMC) by in vitro expansion and interferon (IFN)- intracellular staining. PRINCIPAL FINDINGS: At least three peptides within HCV F protein were identified as HLA-DR or HLA-DP4 presenting epitopes by the proliferation assays in mouse models. Further study with human PBMCs evidenced the specific CD4(+) T cell responses against HCV F protein as well in patients chronically infected with HCV. CONCLUSION: The current study provided the evidence for the first time that HCV F protein could elicit specific CD4(+) T cell response, which may provide an insight into the immunopathogenesis during HCV chronic infection.
Affiliations:Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Reference Type:Literature
PubMed ID:21151917
Journal:PLoS One
Journal Volume:5
Article Pages:e14237
Journal ISSN:1932-6203
Article Chemical List:Epitopes;HLA Antigens;Viral Fusion Proteins;Interferon-gamma
Article MeSH List:Amino Acid Sequence; Animals; CD4-Positive T-Lymphocytes(metabolism); Epitopes(chemistry); HLA Antigens(metabolism); Hepacivirus(metabolism); Hepatitis C(virology); Humans; Interferon-gamma(metabolism); Leukocytes, Mononuclear(metabolism); Mice; Mice, Transgenic; Molecular Sequence Data; Spleen(cytology); Viral Fusion Proteins(metabolism)
Curation Last Updated:2016-01-08 21:51:51