Epitopes described in "Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells."

Article Authors:Sabrina Hoffmann; Babak H Hosseini; Markus Hecker; Ilia Louban; Nadja Bulbuc; Natalio Garbi; Guido H Wabnitz; Yvonne Samstag; Joachim P Spatz; Günter J Hämmerling
Article Title:Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells.
Reference Detail
Reference ID:1021684
Abstract:T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune synapse formation, as determined by LFA-1 and TCR enrichment at the interface of T-cell/APC conjugates using high throughput multispectral imaging flow cytometry. Together, these results suggest that ICAM-1/LFA-1 redistribution to the contact area is mainly responsible for development of strong interaction forces. High forces will keep T-cells and APCs in tight contact, thereby providing a platform for optimal interaction between TCRs and peptide-MHC complexes.
Affiliations:Division of Molecular Immunology, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany.
Reference Type:Literature
PubMed ID:21112354
Journal:Immunol Lett
Journal Volume:136
Article Pages:13-20
Journal ISSN:0165-2478
Article Chemical List:Peptides;Intercellular Adhesion Molecule-1
Article MeSH List:Antigen-Presenting Cells(immunology); Cell Line; Intercellular Adhesion Molecule-1(immunology); Microscopy, Atomic Force; Myelin Sheath(immunology); Peptides(immunology); Spectrophotometry; T-Lymphocytes(immunology)
Curation Last Updated:2016-04-05 22:57:24