Epitopes described in "A multimeric immunogen for the induction of immune memory to beta-amyloid."

Article Authors:Francesca Mantile; Carla Basile; Valeria Cicatiello; Diana De Falco; Antonella Caivano; Piergiuseppe De Berardinis; Antonella Prisco
Article Title:A multimeric immunogen for the induction of immune memory to beta-amyloid.
Reference Detail
Reference ID:1022207
Abstract:The development of active immunotherapy for Alzheimer's disease (AD) requires the identification of immunogens that can ensure a high titer antibody response toward beta-amyloid, whereas minimizing the risks of a cell-mediated adverse reaction. We describe here two novel anti-beta-amyloid vaccines that consist of 'virus like particles' formed by a domain of the bacterial protein E2 that is able to self-assemble into a 60-mer peptide. Peptides 1-11 and 2-6 of beta-amyloid were displayed as N terminal fusions on the surface of the E2 particles. E2-based vaccines induced a fast-rising, robust and persistent antibody response to beta-amyloid in all vaccinated mice. The immune memory induced by a single administration of vaccine (1-11) E2 can be rapidly mobilized by a single booster injection, leading to a very high serum concentration of anti-beta-amyloid antibodies (above 1 mg ml(-1)). E2 vaccination polarizes the immune response toward the production of the anti-inflammatory cytokine interleukin-4 and does not induce a T cell response to beta-amyloid. Thus, E2-based vaccines are promising candidates for the development of immunotherapy protocols for AD.
Affiliations:Istituto di Genetica e Biofisica ABT, CNR, Naples, Italy.
Reference Type:Literature
PubMed ID:21102534
Journal:Immunol Cell Biol
Journal Volume:89
Article Pages:604-9
Journal ISSN:1440-1711
Article Chemical List:Amyloid beta-Peptides;Antibodies, Monoclonal;Epitopes;Immunoglobulin G;Vaccines, Synthetic;Vaccines, Virus-Like Particle;Interleukin-4;Interferon-gamma
Article MeSH List:Alzheimer Disease(immunology); Amyloid beta-Peptides(immunology); Animals; Antibodies, Monoclonal(immunology); Antibody Formation(immunology); Cells, Cultured; Epitopes(immunology); Immunization; Immunoglobulin G(immunology); Immunologic Memory(immunology); Interferon-gamma(biosynthesis); Interleukin-4(biosynthesis); Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Vaccines, Synthetic(immunology); Vaccines, Virus-Like Particle(administration & dosage; immunology)
Curation Last Updated:2015-07-30 20:39:52