Epitopes described in "Hepatitis C virus-specific CD8+ T cell frequencies are associated with the responses of pegylated interferon-α and ribavirin combination therapy in patients with chronic hepatitis C virus infection."

Reference
Article Authors:Tomohide Tatsumi; Tetsuo Takehara; Takuya Miyagi; Shoichi Nakazuru; Eiji Mita; Tatsuya Kanto; Naoki Hiramatsu; Norio Hayashi
Article Title:Hepatitis C virus-specific CD8+ T cell frequencies are associated with the responses of pegylated interferon-α and ribavirin combination therapy in patients with chronic hepatitis C virus infection.
Reference Detail
Reference ID:1021184
Abstract:Aim:  Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes (CTLs) play critical roles in elimination of the HCV-infected hepatocytes. However, the mechanism of HCV elimination by pegylated interferon- (peg-IFN) plus ribavirin is not fully understood. We examined HCV-specific CTL responses during this combination therapy. Methods:  CD8+ T cells were isolated from 16 HCV infected patients treated by this combination therapy and were subjected to IFN- enzyme-linked immunospot (ELISPOT) assay. Results:  The numbers of IFN- spots against HCV Core or NS3 protein-derived peptides in HCV patients before treatment were similar to those in healthy donors, and those in HCV patients significantly increased 4 weeks after the initiation of combination therapy. All HCV Core or NS3 proteins-derived peptides specific CD8+ T cells responses in pre-treated patients were not associated with ALT levels and HCV viral loads of HCV patients before treatment. And those in pre-treated patients were similar between sustained virologic responder (SVR) patients and non-SVR patients. Significant increase of HCV Core or NS3 proteins-derived peptides specific CD8+ T cells responses between before and 4 weeks after this combination therapy were observed in SVR patients, but not in non-SVR patients. Conclusions:  These results demonstrated that significant increase of HCV-specific CD8+ T cells at 4 weeks after the initiation of IFN treatment might be associated with the elimination of HCV. Our findings suggest that the reactivity against HCV Core and NS3 proteins-derived peptides might be useful in predicting the clinical outcome of the combination therapy of peg-IFN and ribavirin.
Affiliations:Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, Suita Department of Gastroenterology, National Hospital Organization Osaka National Hospital, Chuo-ku, Japan Kansai-Rosai Hospital, Amagasaki, Hyogo, Japan.
Date:2011
Reference Type:Literature
PubMed ID:21040277
Journal:Hepatol Res
Journal Volume:41
Article Pages:30-8
Journal ISSN:1386-6346
Curation Last Updated:2014-10-03 22:15:42