Epitopes described in "Programmed Death-1 expression on Epstein Barr virus specific CD8+ T cells varies by stage of infection, epitope specificity, and T-cell receptor usage."

Article Authors:Thomas C Greenough; Shalyn C Campellone; Robin Brody; Surbhi Jain; Victor Sanchez-Merino; Mohan Somasundaran; Katherine Luzuriaga
Article Title:Programmed Death-1 expression on Epstein Barr virus specific CD8+ T cells varies by stage of infection, epitope specificity, and T-cell receptor usage.
Reference Detail
Reference ID:1020847
Abstract:BACKGROUND: Programmed Death-1 (PD-1) is an inhibitory member of the CD28 family of molecules expressed on CD8+ T cells in response to antigenic stimulation. To better understand the role of PD-1 in antiviral immunity we examined the expression of PD-1 on Epstein-Barr virus (EBV) epitope-specific CD8+ T cells during acute infectious mononucleosis (AIM) and convalescence. METHODOLOGY/PRINCIPAL FINDINGS: Using flow cytometry, we observed higher frequencies of EBV-specific CD8+ T cells and higher intensity of PD-1 expression on EBV-specific CD8+ T cells during AIM than during convalescence. PD-1 expression during AIM directly correlated with viral load and with the subsequent degree of CD8+ T cell contraction in convalescence. Consistent differences in PD-1 expression were observed between CD8+ T cells with specificity for two different EBV lytic antigen epitopes. Similar differences were observed in the degree to which PD-1 was upregulated on these epitope-specific CD8+ T cells following peptide stimulation in vitro. EBV epitope-specific CD8+ T cell proliferative responses to peptide stimulation were diminished during AIM regardless of PD-1 expression and were unaffected by blocking PD-1 interactions with PD-L1. Significant variability in PD-1 expression was observed on EBV epitope-specific CD8+ T cell subsets defined by V-beta usage. CONCLUSIONS/SIGNIFICANCE: These observations suggest that PD-1 expression is not only dependent on the degree of antigen presentation, but also on undefined characteristics of the responding cell that segregate with epitope specificity and V-beta usage.
Affiliations:Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America. thomas.greenough@umassmed.edu.
Reference Type:Literature
PubMed ID:20886079
Journal:PLoS One
Journal Volume:5
Article Pages:e12926
Journal ISSN:1932-6203
Article Chemical List:Antigens, CD;Apoptosis Regulatory Proteins;PDCD1 protein, human;Programmed Cell Death 1 Receptor;Receptors, Antigen, T-Cell
Article MeSH List:Antigens, CD(genetics; immunology); Apoptosis Regulatory Proteins(genetics; immunology); CD8-Positive T-Lymphocytes(immunology; virology); Cells, Cultured; Gene Expression; Herpesvirus 4, Human(immunology; physiology); Humans; Infectious Mononucleosis(genetics; immunology; virology); Programmed Cell Death 1 Receptor; Receptors, Antigen, T-Cell(genetics; immunology); Up-Regulation
Curation Last Updated:2015-06-05 02:28:12