Epitopes described in "Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells."

Article Authors:Jeremiah R McDole; Steve C Danzer; Raymund Y K Pun; Yi Chen; Holly L Johnson; Istvan Pirko; Aaron J Johnson
Article Title:Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
Reference Detail
Reference ID:1020908
Abstract:A fundamental question in neuroimmunology is the extent to which CD8 T cells actively engage virus-infected neurons. In the Theiler's murine encephalomyelitis virus (TMEV) model of multiple sclerosis, an effective central nervous system (CNS)-infiltrating antiviral CD8 T cell response offers protection from this demyelinating disease. However, the specific CNS cell types engaged by these protective CD8 T cells in TMEV-resistant strains remains unknown. We used confocal microscopy to visualize the morphology, migration, and specific cellular interactions between adoptively transferred CD8 T cells and specific CNS cell types. Adoptively transferred GFP+ CD8+ splenocytes migrated to the brain and became 93% specific for the immunodominant virus epitope D(b):VP2(121-130). These CD8 T cells also polarized T cell receptor, CD8 protein, and granzyme B toward target neurons. Furthermore, we observed CD8 T cells forming cytoplasmic processes up to 45 m in length. Using live tissue imaging, we determined that these T cell-extended processes (TCEPs) could be rapidly formed and were associated with migratory behavior through CNS tissues. These studies provide evidence that antiviral CD8 T cells have the capacity to engage virus-infected neurons in vivo and are the first to document and measure the rapid formation of TCEPs on these brain-infiltrating lymphocytes using live tissue imaging.
Reference Type:Literature
PubMed ID:20813972
Journal:Am J Pathol
Journal Volume:177
Article Pages:1823-33
Journal ISSN:1525-2191
Article Chemical List:Epitopes, T-Lymphocyte
Article MeSH List:Adoptive Transfer; Animals; Brain(immunology; virology); CD8-Positive T-Lymphocytes(immunology; metabolism); Cardiovirus Infections(immunology; virology); Central Nervous System(immunology; virology); Central Nervous System Viral Diseases(immunology; virology); Epitopes, T-Lymphocyte(immunology); Female; Immunoenzyme Techniques; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Neurons(immunology; virology); Theilovirus(immunology)
Curation Last Updated:2015-07-30 20:37:42