Epitopes described in "Using T. brucei as a biological epitope-display platform to elicit specific antibody responses."

Article Authors:Pete Stavropoulos; F Nina Papavasiliou
Article Title:Using T. brucei as a biological epitope-display platform to elicit specific antibody responses.
Reference Detail
Reference ID:1021232
Abstract:The African trypanosome (Trypanosoma brucei) is transmitted by the bite of the tsetse vector to the mammalian bloodstream where it exists as a completely extracellular parasite. As a result of this exposure, the parasite elicits a robust immune response that is almost exclusively antibody mediated, and is extremely specific to the trypanosome coat displayed on the surface. This coat is comprised of ~11 million copies of a single gpi-linked molecule (the variable surface glycoprotein or VSG) and can therefore be used as a powerful platform for the immunogenic display of antigenic determinants. Here we describe a method to display repetitive, ordered arrays of linear epitopes on the surface of T. brucei and to then use the engineered organisms to generate specific anti-epitope antibody responses, upon injection into mice. This method offers an alternative approach to generating anti-peptide antibodies, and could be a useful option in cases where more traditional methods have failed.
Affiliations:Laboratory of Lymphocyte Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. stavrop@mail.rockefeller.edu.
Reference Type:Literature
PubMed ID:20800596
Journal:J Immunol Methods
Journal Volume:362
Article Pages:190-4
Journal ISSN:0022-1759
Article Chemical List:Antibodies, Protozoan;Epitopes;Membrane Proteins;Protozoan Proteins
Article MeSH List:Animals; Antibodies, Protozoan(immunology); Epitope Mapping(methods); Epitopes(genetics; immunology); Membrane Proteins(genetics; immunology); Mice; Protozoan Proteins(genetics; immunology); Trypanosoma brucei brucei(genetics; immunology); Trypanosomiasis, African(genetics; immunology)
Curation Last Updated:2015-06-05 02:31:56