Epitopes described in "Diversity in CD8(+) T cell function and epitope breadth among persons with genital herpes."

Article Authors:Kerry J Laing; Amalia S Magaret; Dawn E Mueller; Lin Zhao; Christine Johnston; Stephen C De Rosa; David M Koelle; Anna Wald; Lawrence Corey
Article Title:Diversity in CD8(+) T cell function and epitope breadth among persons with genital herpes.
Reference Detail
Reference ID:1021967
Abstract:CD8(+) T cells are known to be important in clearing herpes simplex virus (HSV) infections. However, investigating the specific antiviral mechanisms employed by HSV-2-specific T cell populations is limited by a lack of reagents such as CD8(+) T cell epitopes and specific tetramers. Using a combination of intracellular cytokine staining flow cytometry and ELISpot methods, we functionally characterized peripheral HSV-2-specific CD8(+) T cells from peripheral blood mononuclear cell (PBMC) that recognize 14 selected HSV-2 open-reading frames (ORFs) from 55 HSV-2 seropositive persons; within these ORFs, we subsequently identified more than 20 unique CD8(+) T cell epitopes. CD8(+) T cells to HSV-2 exhibited significant heterogeneity in their functional characteristics, proliferation, production of inflammatory cytokines, and potential to degranulate ex vivo. The diversity in T cell response in these ex vivo assessments offers the potential of defining immune correlates of HSV-2 reactivation in humans.
Affiliations:Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Reference Type:Literature
PubMed ID:20635156
Journal:J Clin Immunol
Journal Volume:30
Article Pages:703-22
Journal ISSN:0271-9142
Article Chemical List:Antigens, Viral;Cytokines;Epitopes, T-Lymphocyte
Article MeSH List:Adult; Aged; Antigenic Variation; Antigens, Viral(immunology); CD8-Positive T-Lymphocytes(immunology; metabolism; pathology; virology); Cell Degranulation(immunology); Cell Proliferation; Cells, Cultured; Cytokines(secretion); Epitope Mapping; Epitopes, T-Lymphocyte(chemistry; immunology; metabolism); Female; Herpes Genitalis(diagnosis; immunology); Herpesvirus 2, Human(immunology; pathogenicity); Humans; Male; Middle Aged
Curation Last Updated:2016-01-15 21:55:21