Epitopes described in "An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus."

Article Authors:Guangyu Zhao; Shihui Sun; Lanying Du; Wenjun Xiao; Zhitao Ru; Zhihua Kou; Yan Guo; Hong Yu; Shibo Jiang; Yuchun Lone; Bo-Jian Zheng; Yusen Zhou
Article Title:An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus.
Reference Detail
Reference ID:1020885
Abstract:BACKGROUND: A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. RESULTS: Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. CONCLUSIONS: Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus.
Affiliations:State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Reference Type:Literature
PubMed ID:20624292
Journal:Virol J
Journal Volume:7
Article Pages:151
Journal ISSN:1743-422X
Article Chemical List:Influenza Vaccines;M2 protein, Influenza A virus;Peptides;Viral Matrix Proteins
Article MeSH List:Animals; China(epidemiology); Cross Protection; Disease Models, Animal; Disease Outbreaks; Female; Humans; Influenza A Virus, H1N1 Subtype(immunology; physiology); Influenza A Virus, H5N1 Subtype(chemistry; immunology); Influenza Vaccines(administration & dosage; immunology); Influenza, Human(epidemiology; immunology; prevention & control; virology); Mice; Mice, Inbred BALB C; Peptides(immunology); Protein Structure, Tertiary; Viral Matrix Proteins(administration & dosage; chemistry; immunology)
Curation Last Updated:2015-06-05 02:28:49