Epitopes described in "Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed mice."

Article Authors:Tinashe B Ruwona; Victor J Johnson; Detlef Schmechel; Reuben H Simoyi; Donald Beezhold; Paul D Siegel
Article Title:Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed mice.
Reference Detail
Reference ID:1019883
Abstract:Toluene diisocyanate (TDI) is an industrially important polymer cross-linker used in the production of polyurethane. Workplace exposure to TDI and other diisocyanates is reported to be a leading cause of low molecular weight-induced occupational asthma (OA). Currently we have a limited understanding of the pathogenesis of OA. Monoclonal antibodies (MAbs) that recognize TDI bound proteins would be valuable tools/reagents, both in exposure monitoring and in TDI-induced asthma research. We sought to develop toluene diisocyanate (TDI)-specific MAbs for potential use in the development of standardized immunoassays for exposure and biomarker assessments. Mice were exposed 4 h/day for 12 consecutive weekdays to 50 ppb, 2,4;2,6 TDI vapor (80/20 mixture). Splenocytes were isolated 24 h after the last exposure for hybridoma production. Hybridomas were screened in a solid-phase indirect enzyme-linked immunosorbent assay (ELISA) against a 2,4 TDI-human serum albumin (2,4 TDI-HSA) protein conjugate. Three hybridomas producing 2,4 TDI-HSA reactive IgM MAbs were obtained. The properties of these MAbs (isotype and reactivity to various protein-isocyanate conjugate epitopes) were characterized using ELISA, dot blot, and Western blot analyses. Western blot analyses demonstrated that some TDI conjugates form inter- and intra-molecular links, resulting in multimers and a change in the electrophoretic mobility of the conjugate. These antibodies may be useful tools for the isolation of endogenous diisocyanate-modified proteins after natural or experimental exposures and for characterization of the toxicity of specific dNCOs.
Affiliations:Allergy and Clinical Immunology, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505-2888, USA.
Reference Type:Literature
PubMed ID:20568997
Journal:Hybridoma (Larchmt)
Journal Volume:29
Article Pages:221-9
Journal ISSN:1554-0014
Article Chemical List:Antibodies, Monoclonal;Haptens;Immunoglobulin M;Isocyanates;Serum Albumin;Toluene 2,4-Diisocyanate
Article MeSH List:Administration, Inhalation; Animals; Antibodies, Monoclonal(classification; diagnostic use; immunology); Antibody Specificity; Asthma(chemically induced; diagnosis); Enzyme-Linked Immunosorbent Assay; Female; Haptens(immunology); Humans; Hybridomas; Immunoblotting; Immunoglobulin M; Isocyanates(immunology); Mice; Mice, Inbred C57BL; Serum Albumin; Toluene 2,4-Diisocyanate(administration & dosage; immunology; toxicity)
Curation Last Updated:2016-04-11 21:11:41