Epitopes described in "Defective cross-presentation of viral antigens in GILT-free mice."

Article Authors:Reshma Singh; Peter Cresswell
Article Title:Defective cross-presentation of viral antigens in GILT-free mice.
Reference Detail
Reference ID:1019734
Abstract:Gamma-interferon-inducible lysosomal thiolreductase (GILT) promotes major histocompatibility complex (MHC) class II-restricted presentation of exogenous antigens containing disulfide bonds. Here, we show that GILT also facilitates MHC class I-restricted recognition of such antigens by CD8+ T cells, or cross-presentation. GILT is essential for cross-presentation of a CD8+ T cell epitope of glycoprotein B (gB) from herpes simplex virus 1 (HSV-1) but not for its presentation by infected cells. Initiation of the gB-specific CD8+ T cell response during HSV-1 infection, or cross-priming, is highly GILT-dependent, as is initiation of the response to the envelope glycoproteins of influenza A virus. Efficient cross-presentation of disulfide-rich antigens requires a complex pathway involving GILT-mediated reduction, unfolding, and partial proteolysis, followed by translocation into the cytosol for proteasomal processing.
Affiliations:Department of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, 300 Cedar Street, Post Office Box 208011, New Haven, CT 06250-8011, USA.
Reference Type:Literature
PubMed ID:20538950
Journal Volume:328
Article Pages:1394-8
Journal ISSN:1095-9203
Article Chemical List:Antigens, Viral;Epitopes, T-Lymphocyte;H-2 Antigens;H-2Kb protein, mouse;Ifi30 protein, mouse;Viral Envelope Proteins;glycoprotein B, Simplexvirus;transporter associated with antigen processing (TAP);Oxidoreductases;Proteasome Endopeptidase Complex
Article MeSH List:ATP-Binding Cassette Transporters(metabolism); Animals; Antigen Presentation; Antigens, Viral(immunology); CD8-Positive T-Lymphocytes(immunology); Cross-Priming; Cytosol(metabolism); Dendritic Cells(immunology); Epitopes, T-Lymphocyte(immunology); H-2 Antigens(immunology); HeLa Cells; Herpes Simplex(immunology; metabolism); Herpesvirus 1, Human(immunology); Humans; Influenza A virus(immunology); Mice; Orthomyxoviridae Infections(immunology); Oxidoreductases(genetics; metabolism); Proteasome Endopeptidase Complex(metabolism); Viral Envelope Proteins(immunology; metabolism)
Curation Last Updated:2015-07-30 20:36:15