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| Article Authors: | Diana L Martin; Kaja Murali-Krishna; Rick L Tarleton |
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| Article Title: | Generation of Trypanosoma cruzi-specific CD8+ T-cell immunity is unaffected by the absence of type I interferon signaling. |
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| Reference ID: | 1019817 |
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| Abstract: | Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) account for approximately 30% of the total CD8+ T-cell population at the peak of infection in C57BL/6 mice. Type I interferons (IFN-I) are pleiotropic cytokines that play a critical role in both innate and adaptive immunity against a variety of infections, but their induction and their role in infection are dictated by the infectious agent. Because type I IFNs and IFN-responsive genes are evident early after T. cruzi infection of host cells, we examined the influence of IFN-I on the development of CD8+ T-cell responses during this infection. Mice lacking the receptor for IFN-I (IFNARKO) and their wild-type counterparts both developed chronic infections and generated similar frequencies of immunodominant TSKB20- and subdominant TSKB18-specific CD8+ T cells following T. cruzi infection. In contrast, peak TSKB20-specific CD8+ T-cell responses generated during infection with vaccinia virus engineered to express TSKB20 were approximately 2.5-fold lower in IFNARKO mice than B6 mice, although after viral clearance, the frequencies of TSKB20-specific CD8+ T cells stabilized at similar levels. Together, these data suggest that IFN-I induction and biology are dependent upon the microbial context and emphasize the need to investigate various infection models for a full understanding of CD8+ T-cell development. |
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| Affiliations: | Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30502, USA. |
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| Date: | 2010 |
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| Reference Type: | Literature |
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| PubMed ID: | 20457790 |
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| Journal: | Infect Immun |
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| Journal Volume: | 78 |
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| Article Pages: | 3154-9 |
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| Journal ISSN: | 1098-5522 |
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| Article Chemical List: | Interferon Regulatory Factors;Interferon Type I;Receptors, Interferon |
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| Article MeSH List: | Animals; CD8-Positive T-Lymphocytes(immunology; physiology); Chagas Disease(immunology); Female; Immunity, Cellular(immunology; physiology); Interferon Regulatory Factors(genetics; immunology; physiology); Interferon Type I(immunology; physiology); Lymphocyte Activation(immunology; physiology); Male; Mice; Mice, Inbred C57BL; Receptors, Interferon(immunology; physiology); Signal Transduction(immunology; physiology); Trypanosoma cruzi(immunology) |
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| Curation Last Updated: | 2010-10-26 03:41:35 |
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