Epitopes described in "Generation of Trypanosoma cruzi-specific CD8+ T-cell immunity is unaffected by the absence of type I interferon signaling."

Article Authors:Diana L Martin; Kaja Murali-Krishna; Rick L Tarleton
Article Title:Generation of Trypanosoma cruzi-specific CD8+ T-cell immunity is unaffected by the absence of type I interferon signaling.
Reference Detail
Reference ID:1019817
Abstract:Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) account for approximately 30% of the total CD8+ T-cell population at the peak of infection in C57BL/6 mice. Type I interferons (IFN-I) are pleiotropic cytokines that play a critical role in both innate and adaptive immunity against a variety of infections, but their induction and their role in infection are dictated by the infectious agent. Because type I IFNs and IFN-responsive genes are evident early after T. cruzi infection of host cells, we examined the influence of IFN-I on the development of CD8+ T-cell responses during this infection. Mice lacking the receptor for IFN-I (IFNARKO) and their wild-type counterparts both developed chronic infections and generated similar frequencies of immunodominant TSKB20- and subdominant TSKB18-specific CD8+ T cells following T. cruzi infection. In contrast, peak TSKB20-specific CD8+ T-cell responses generated during infection with vaccinia virus engineered to express TSKB20 were approximately 2.5-fold lower in IFNARKO mice than B6 mice, although after viral clearance, the frequencies of TSKB20-specific CD8+ T cells stabilized at similar levels. Together, these data suggest that IFN-I induction and biology are dependent upon the microbial context and emphasize the need to investigate various infection models for a full understanding of CD8+ T-cell development.
Affiliations:Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30502, USA.
Reference Type:Literature
PubMed ID:20457790
Journal:Infect Immun
Journal Volume:78
Article Pages:3154-9
Journal ISSN:0019-9567
Article Chemical List:Interferon Regulatory Factors;Interferon Type I;Receptors, Interferon
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(immunology; physiology); Chagas Disease(immunology); Female; Immunity, Cellular(immunology; physiology); Interferon Regulatory Factors(genetics; immunology; physiology); Interferon Type I(immunology; physiology); Lymphocyte Activation(immunology; physiology); Male; Mice; Mice, Inbred C57BL; Receptors, Interferon(immunology; physiology); Signal Transduction(immunology; physiology); Trypanosoma cruzi(immunology)
Curation Last Updated:2015-07-30 20:36:29