Epitopes described in "Ribosomal P autoantibodies are present before SLE onset and are directed against non-C-terminal peptides."

Article Authors:Latisha D Heinlen; Lauren L Ritterhouse; Micah T McClain; Michael P Keith; Barbara R Neas; John B Harley; Judith A James
Article Title:Ribosomal P autoantibodies are present before SLE onset and are directed against non-C-terminal peptides.
Reference Detail
Reference ID:1019955
Abstract:Autoantibodies to ribosomal P (ribo P) are found in 15-30% of systemic lupus erythematosus (SLE) patients and are highly specific for SLE. The goal of this study is to assess the temporal association of anti-ribosomal P (anti-P) responses with SLE disease onset, as well as to characterize select humoral ribo P epitopes targeted in early, pre-diagnostic SLE samples. Patients with stored serial serum samples available prior to SLE diagnosis were identified from a military cohort. Each sample was tested for antibodies against ribo P utilizing standard C terminus ribo P enzyme-linked immunosorbent assays (ELISA) and a solid phase, bead-based assay with affinity-purified ribo P proteins. In this study, antibodies to ribo P were more common in African American SLE patients (p = 0.026), and anti-P-positive patients comprised a group with more measured autoantibody specificities than did other SLE patients (3.5 vs 2.2, p < 0.05). Antibodies against ribo P were present on average 1.7 years before SLE diagnosis and were detected an average of 1.08 years earlier in pre-diagnostic SLE samples using affinity-purified whole protein rather than C-terminal peptide alone (p = 0.0019). Furthermore, 61% of anti-P-positive patients initially had antibodies to aa 99-113, a known ribosomal P0 antigenic target, at a time point when no antibodies to the clinically used C terminus were detected. Our findings provide evidence that antibodies against ribosomal P frequently develop before clinical SLE diagnosis and are more broadly reactive than previously thought by targeting regions outside of the C terminus.
Affiliations:Oklahoma Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104, USA.
Reference Type:Literature
PubMed ID:20396862
Journal:J Mol Med (Berl)
Journal Volume:88
Article Pages:719-27
Journal ISSN:1432-1440
Article Chemical List:Autoantibodies;Autoantigens;Epitopes;Peptide Fragments;Ribosomal Proteins
Article MeSH List:Adolescent; Adult; African Americans; Autoantibodies(blood; immunology); Autoantigens(blood; immunology); Epitopes(immunology); Female; Humans; Lupus Erythematosus, Systemic(blood; diagnosis; immunology); Male; Middle Aged; Peptide Fragments(immunology); Ribosomal Proteins(immunology); Young Adult
Curation Last Updated:2015-06-05 02:26:02