Epitopes described in "Detailed characterization of T cell responses to herpes simplex virus-2 in immune seronegative persons."

Article Authors:Christine M Posavad; Michael Remington; Dawn E Mueller; Lin Zhao; Amalia S Magaret; Anna Wald; Lawrence Corey
Article Title:Detailed characterization of T cell responses to herpes simplex virus-2 in immune seronegative persons.
Reference Detail
Reference ID:1019099
Abstract:In 2003, we described a small cohort of subjects (n = 6) who possessed no detectable serum Abs to HSV-1 or HSV-2 and no clinical or virological evidence of mucosal HSV infection yet possessed consistently detectable HSV-specific T cell responses measured primarily by lymphoproliferative (LP) and CTL assays to whole HSV-2 Ag. We termed these persons immune seronegative (IS). This report characterizes the T cell responses in 22 IS subjects largely recruited from studies of HSV-seronegative subjects in ongoing sexual relationships with HSV-2-seropositive (HSV-2(+)) partners using pools of overlapping peptides spanning 16 immuno-prevalent HSV-2 proteins. Overall, 77% of IS subjects had HSV-specific LP responses, 85% had IFN-gamma ELISPOT responses to at least one HSV-2 peptide pool, and 55% had both LP and IFN-gamma ELISPOT responses. In some cases, IFN-gamma ELISPOT responses were in excess of 500 spot-forming cells per 10(6) PBMCs and persisted for over 5 y. Although HSV-2(+) subjects (n = 40) had frequent responses to glycoproteins and tegument and immediate-early (IE) proteins of HSV-2, T cell responses in IS subjects were directed primarily at UL39 and the IE proteins ICP4 and ICP0. These data suggest that the antigenic repertoire of T cells in IS subjects is skewed compared with that of HSV-2(+) subjects and that IS subjects had more frequent T cell responses to IE proteins and infrequent T cell responses to virion components. Understanding the mechanism(s) by which such responses are elicited may provide important insights in developing novel strategies for preventing acquisition of sexually acquired HSV-2.
Affiliations:Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109, USA. posavad@u.washington.edu.
Reference Type:Literature
PubMed ID:20164419
Journal:J Immunol
Journal Volume:184
Article Pages:3250-9
Journal ISSN:0022-1767
Article Chemical List:Antibodies, Viral;Epitopes, T-Lymphocyte
Article MeSH List:Adult; Aged; Amino Acid Sequence; Antibodies, Viral(blood); Clone Cells; Cohort Studies; Cytotoxicity Tests, Immunologic; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte(immunology); Female; Herpes Genitalis(immunology; transmission; virology); Herpesvirus 2, Human(immunology); Humans; Male; Middle Aged; Molecular Sequence Data; Risk Factors; T-Lymphocytes(immunology; virology); Young Adult
Curation Last Updated:2015-06-05 01:59:36