Epitopes described in "Mapping epitopes of the Plasmodium vivax Duffy binding protein with naturally acquired inhibitory antibodies."

Article Authors:Patchanee Chootong; Francis B Ntumngia; Kelley M VanBuskirk; Jia Xainli; Jennifer L Cole-Tobian; Christopher O Campbell; Tresa S Fraser; Christopher L King; John H Adams
Article Title:Mapping epitopes of the Plasmodium vivax Duffy binding protein with naturally acquired inhibitory antibodies.
Reference Detail
Reference ID:1026370
Abstract:Plasmodium vivax Duffy binding protein (DBP) is a merozoite microneme ligand vital for blood-stage infection, which makes it an important candidate vaccine for antibody-mediated immunity against vivax malaria. A differential screen with a linear peptide array compared the reactivities of noninhibitory and inhibitory high-titer human immune sera to identify target epitopes associated with protective immunity. Naturally acquired anti-DBP-specific serologic responses observed in the residents of a region of Papua New Guinea where P. vivax is highly endemic exhibited significant changes in DBP-specific titers over time. The anti-DBP functional inhibition for each serum ranged from complete inhibition to no inhibition even for high-titer responders to the DBP, indicating that epitope specificity is important. Inhibitory immune human antibodies identified specific B-cell linear epitopes on the DBP (SalI) ligand domain that showed significant correlations with inhibitory responses. Affinity-purified naturally acquired antibodies on these epitopes inhibited the DBP erythrocyte binding function greatly, confirming the protective value of specific epitopes. These results represent an important advance in our understanding of part of blood-stage immunity to P. vivax and some of the specific targets for vaccine-elicited antibody protection.
Affiliations:Global Health Infectious Disease Research Program, University of South Florida, Tampa, Florida, USA.
Reference Type:Literature
PubMed ID:20008533
Journal:Infect Immun
Journal Volume:78
Article Pages:1089-95
Journal ISSN:0019-9567
Article Chemical List:Antibodies, Neutralizing;Antibodies, Protozoan;Antigens, Protozoan;Duffy antigen binding protein, Plasmodium;Epitopes, B-Lymphocyte;Protozoan Proteins;Receptors, Cell Surface
Article MeSH List:Adolescent; Adult; Aged; Amino Acid Sequence; Animals; Antibodies, Neutralizing(immunology; isolation & purification); Antibodies, Protozoan(immunology; isolation & purification); Antigens, Protozoan(chemistry; immunology); Child; Epitope Mapping; Epitopes, B-Lymphocyte(immunology); Humans; Middle Aged; Models, Molecular; Molecular Sequence Data; Papua New Guinea; Plasmodium vivax(immunology); Protein Array Analysis; Protein Structure, Tertiary; Protozoan Proteins(chemistry; immunology); Receptors, Cell Surface(chemistry; immunology); Young Adult
Curation Last Updated:2015-06-05 04:03:41