Epitopes described in "Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization."

Article Authors:Mary Dawn T Co; Elizabeth D Kilpatrick; Alan L Rothman
Article Title:Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization.
Reference Detail
Reference ID:1016563
Abstract:Management of yellow fever is focused on the prevention of illness by the use of the yellow fever virus (YFV) 17D vaccine. The role of neutralizing antibodies in protection is generally accepted with YFV-specific T cells likely contributing to the control of viral replication. We studied CD8(+) T-cell responses to four defined human leucocyte antigen-B35-restricted epitopes in YFV vaccine recipients as a model of the kinetics of cytotoxic T-lymphocyte responses to an acute human viral infection. Multiple features of these epitope-specific responses were analysed after vaccination including magnitude, cytokine production, phenotype and T-cell receptor repertoire. Peak peptide-specific interferon-gamma (IFN-gamma) responses of almost 1% of CD8(+) T cells were seen as early as 2 weeks post-vaccination; however, dominant responses varied between donors. Peptide-specific responses were still detectable at 54 months post-vaccination. Tetramer-positive cells, at high frequencies, were detected as early as 7-9 days, before detectable IFN-gamma-producing cells, suggesting a defect in the functional capacity of some antigen-specific cells early post-vaccination. The predominant memory phenotype of the tetramer-positive population was a differentiated effector (CD45RA(+) CCR7(-) CD62L(-)) phenotype. The T-cell receptor Vbeta analysis revealed a diverse oligoclonal repertoire in tetramer-positive T-cell populations in two individuals. These characteristics of the YFV-specific T-cell response could contribute to vaccine effectiveness.
Affiliations:Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA. mary.co@umassmed.edu.
Reference Type:Literature
PubMed ID:19740333
Journal Volume:128
Article Pages:e718-27
Journal ISSN:0019-2805
Article Chemical List:Antibodies, Neutralizing;Cytokines;HLA-B35 Antigen;NS1 protein, Flavivirus;Viral Envelope Proteins;Viral Nonstructural Proteins;Yellow Fever Vaccine;yellow fever virus envelope protein E;Interferon-gamma
Article MeSH List:Antibodies, Neutralizing(blood; immunology); CD8-Positive T-Lymphocytes(immunology); Cytokines(immunology); HLA-B35 Antigen(immunology); Humans; Interferon-gamma(biosynthesis; immunology); Kinetics; Vaccination; Viral Envelope Proteins(immunology); Viral Nonstructural Proteins(immunology); Yellow Fever(immunology; prevention & control); Yellow Fever Vaccine(immunology); Yellow fever virus(immunology)
Curation Last Updated:2015-06-05 01:46:44