Epitopes described in "Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad spectrum of CD4(+) T-cell responses to internal proteins, hemagglutinins, and neuraminidases."

Article Authors:Jenny Aurielle B Babon; John Cruz; Laura Orphin; Pamela Pazoles; Mary Dawn T Co; Francis A Ennis; Masanori Terajima
Article Title:Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad spectrum of CD4(+) T-cell responses to internal proteins, hemagglutinins, and neuraminidases.
Reference Detail
Reference ID:1019592
Abstract:We performed a genome-wide screening for T-cell epitopes using synthetic peptides that encompass all of the influenza A viral proteins, including subtype variants for hemagglutinin (HA; H1, H3, and H5) and neuraminidase (NA; human and avian N1 and N2) proteins, based on the sequence information of recently circulating strains. We identified a total of 83 peptides, 54 of them novel, to which specific T cells were detectable in interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot assays using peripheral blood mononuclear cells from four healthy adult donors. The surface glycoproteins, HA and NA, major components of vaccines, expressed many T-cell epitopes. HA and matrix protein 1 expressed more T-cell epitopes than other viral proteins, most of which were recognized by CD4(+) T cells. We established several cytotoxic CD4(+) T-cell lines from these donors. We also analyzed H1 and H3 HA-specific T-cell responses using the peripheral blood mononuclear cells of 30 hospital workers. Fifty-three percent of donors gave a positive response to H3 HA peptides, whereas 17% gave a positive response to H1 HA peptides. Our genome-wide screening is useful in identifying T-cell epitopes and is complementary to the approach based on the predicted binding peptides to well-studied HLA-A, -B, and -DR alleles.
Affiliations:Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Reference Type:Literature
PubMed ID:19524006
Journal:Hum Immunol
Journal Volume:70
Article Pages:711-21
Journal ISSN:0198-8859
Article Chemical List:Epitopes, T-Lymphocyte;Hemagglutinins;M1 protein, Influenza A virus;Peptide Fragments;Viral Matrix Proteins;Viral Proteins;Interferon-gamma;Neuraminidase
Article MeSH List:Adult; CD4-Positive T-Lymphocytes(immunology; metabolism; pathology); Cell Line; Cross Reactions; Cytotoxicity, Immunologic; Epitope Mapping; Epitopes, T-Lymphocyte(chemistry; immunology; metabolism); Genome, Viral(immunology); Genome-Wide Association Study; Hemagglutinins(chemistry; immunology; metabolism); Histocompatibility Testing; Humans; Influenza A Virus, H5N1 Subtype(immunology); Interferon-gamma(secretion); Neuraminidase(chemistry; immunology; metabolism); Peptide Fragments(chemistry; immunology; metabolism); Viral Matrix Proteins(chemistry; immunology; metabolism); Viral Proteins(chemistry; immunology; metabolism)
Curation Last Updated:2015-06-05 02:09:57