Epitopes described in "Resistance to celiac disease in humanized HLA-DR3-DQ2-transgenic mice expressing specific anti-gliadin CD4+ T cells."

Article Authors:Andrea L de Kauwe; Zhenjun Chen; Robert P Anderson; Catherine L Keech; Jason D Price; Odilia Wijburg; David C Jackson; Jodi Ladhams; Janette Allison; James McCluskey
Article Title:Resistance to celiac disease in humanized HLA-DR3-DQ2-transgenic mice expressing specific anti-gliadin CD4+ T cells.
Reference Detail
Reference ID:1014858
Abstract:Celiac disease is a chronic inflammatory enteropathy caused by cellular immunity to dietary gluten. More than 90% of patients carry HLA-DQ2 encoded by HLA-DQA1*05 and DQB1*02, and gluten-specific CD4(+) T cells from intestinal biopsies of these patients are HLA-DQ2-restricted, produce Th1 cytokines and preferentially recognize gluten peptides deamidated by tissue transglutaminase. We generated mice lacking murine MHC class II genes that are transgenic for human CD4 and the autoimmunity and celiac disease-associated HLA-DR3-DQ2 haplotype. Immunization with the alpha-gliadin 17-mer that incorporates the overlapping DQ2-alpha-I and DQ2-alpha-II epitopes immunodominant in human celiac disease generates peptide-specific HLA-DQ2-restricted CD4(+) T cells. When exposed to dietary gluten, naive or gliadin-primed mice do not develop pathology. Coincident introduction of dietary gluten and intestinal inflammation resulted in low-penetrance enteropathy and tissue transglutaminase-specific IgA. Two further strains of transgenic mice expressing HLA-DR3-DQ2 and human CD4, one with a NOD background and another TCR transgenic having over 90% of CD4(+) T cells specific for the DQ2-alpha-II epitope with a Th1 phenotype, were also healthy when consuming gluten. These humanized mouse models indicate that gluten ingestion can be tolerated without intestinal pathology even when HLA-DQ2-restricted CD4(+) T cell immunity to gluten is established, thereby implicating additional factors in controlling the penetrance of celiac disease.
Affiliations:Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia.
Reference Type:Literature
PubMed ID:19494267
Journal:J Immunol
Journal Volume:182
Article Pages:7440-50
Journal ISSN:1550-6606
Article Chemical List:Epitopes, T-Lymphocyte;FOXP3 protein, human;Forkhead Transcription Factors;HLA-DQ Antigens;HLA-DR3 Antigen;Receptors, Antigen, T-Cell;Gliadin
Article MeSH List:Animals; CD4-Positive T-Lymphocytes(immunology; metabolism); Celiac Disease(genetics; immunology; metabolism; pathology); Cell Separation; Cells, Cultured; Epitopes, T-Lymphocyte(immunology); Forkhead Transcription Factors(immunology); Gliadin(immunology); HLA-DQ Antigens(genetics; immunology; metabolism); HLA-DR3 Antigen(genetics; immunology; metabolism); Health; Hybridomas; Mice; Mice, Inbred C57BL; Mice, Transgenic; Receptors, Antigen, T-Cell(immunology); Substrate Specificity
Curation Last Updated:2014-10-03 21:34:10