Epitopes described in "Specific recognition of the C-terminal end of A beta 42 by a high affinity monoclonal antibody."

Article Authors:Trine Veje Axelsen; Arne Holm; Svend Birkelund; Gunna Christiansen; Michael Ploug; Ida Elisabeth Holm
Article Title:Specific recognition of the C-terminal end of A beta 42 by a high affinity monoclonal antibody.
Reference Detail
Reference ID:1014977
Abstract:The neurotoxic peptide A beta(42) is derived from the amyloid precursor protein by proteolytic cleavage and is deposited in the brain of patients suffering from Alzheimer's disease (AD). In this study we generate a high affinity monoclonal antibody that targets the C-terminal end of A beta(42) with high specificity. By this is meant that the paratope of the antibody must enclose the C-terminal end of A beta(42) including the carboxy-group of amino acid 42, and not just recognize a linear epitope in the C-terminal part of A beta. This has been accomplished by using a unique antigen construct made by the Ligand Presenting Assembly technology (LPA technology). This strategy results in dimeric presentation of the free C-terminal end of A beta(42). The generated Mab A beta1.1 is indeed specific for the C-terminal end of A beta(42) to which it binds with high affinity. Mab A beta1.1 recognizes the epitope in human AD tissue and stains plaques with high specificity. Therefore the monoclonal antibody can thus be useful in the histological investigations of the AD pathology.
Affiliations:Clinical Institute, The Faculty of Health Science, Aarhus University, Brendstrupgårdsvej 100, 8200 Arhus N, Denmark. trine@loke.dk
Reference Type:Literature
PubMed ID:19447496
Journal:Mol Immunol
Journal Volume:46
Article Pages:2267-73
Journal ISSN:0161-5890
Article Chemical List:Amyloid beta-Peptides;Antibodies, Monoclonal;Peptide Fragments;amyloid beta-protein (1-42)
Article MeSH List:Alzheimer Disease(metabolism; pathology); Amyloid beta-Peptides(immunology; metabolism); Antibodies, Monoclonal(immunology); Antibody Affinity; Brain(metabolism; pathology); Humans; Immunohistochemistry; Peptide Fragments(immunology; metabolism); Protein Multimerization
Curation Last Updated:2014-10-03 21:34:58