Epitopes described in "H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4."

Article Authors:Junbao Yang; John A Gebe; Laurie Huston; Eddie James; Venus Tan; Betty B Yue; Gerald T Nepom; William W Kwok
Article Title:H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4.
Reference Detail
Reference ID:1018856
Abstract:CD4+ T cells play a pivotal role in the viral immunity, and as such identification of unique strain-specific HLA class II restricted epitopes is essential for monitoring cellular strain-specific viral immunity. Using Tetramer-Guided Epitope Mapping technique, we identified HLA-DR0401 restricted HA epitopes that are strain-specific to H5N1 virion. Two immunodominant epitopes H5HA(441-460) and H5HA(57-76) were identified from in vitro stimulated human PBMC. Both epitopes elicit strong cellular immune responses when HLA-DR0401 transgenic mice are immunized with H5N1 subvirion indicating in vivo naturally processed immunodominant epitopes. The H5HA(57-76) epitope is unique for the H5N1 strain but conserved among all H5N1 clades recommended for vaccine development by World Health Organization. The unique H5HA(57-76) response was uncommon in unexposed individuals and only observed in the naïve T cell subset. Thus, H5N1 strain-specific H5HA(57-76) immunogenic epitope represents a unique marker for monitoring the efficacy of vaccination or as a candidate vaccine peptide.
Affiliations:Benaroya Research Institute at Virginia Mason, Seattle, WA 98101-2795, United States.
Reference Type:Literature
PubMed ID:19446935
Journal Volume:27
Article Pages:3862-9
Journal ISSN:0264-410X
Article Chemical List:Epitopes, T-Lymphocyte;HLA-DR4 Antigen;Hemagglutinin Glycoproteins, Influenza Virus;hemagglutinin, avian influenza A virus
Article MeSH List:Animals; CD4-Positive T-Lymphocytes(immunology); Cells, Cultured; Epitope Mapping; Epitopes, T-Lymphocyte(immunology); HLA-DR4 Antigen(immunology); Hemagglutinin Glycoproteins, Influenza Virus(immunology); Humans; Influenza A Virus, H5N1 Subtype(immunology); Mice; Mice, Transgenic
Curation Last Updated:2015-06-16 21:27:44