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| Article Authors: | Junbao Yang; John A Gebe; Laurie Huston; Eddie James; Venus Tan; Betty B Yue; Gerald T Nepom; William W Kwok |
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| Article Title: | H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4. |
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| Reference ID: | 1018856 |
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| Abstract: | CD4+ T cells play a pivotal role in the viral immunity, and as such identification of unique strain-specific HLA class II restricted epitopes is essential for monitoring cellular strain-specific viral immunity. Using Tetramer-Guided Epitope Mapping technique, we identified HLA-DR0401 restricted HA epitopes that are strain-specific to H5N1 virion. Two immunodominant epitopes H5HA(441-460) and H5HA(57-76) were identified from in vitro stimulated human PBMC. Both epitopes elicit strong cellular immune responses when HLA-DR0401 transgenic mice are immunized with H5N1 subvirion indicating in vivo naturally processed immunodominant epitopes. The H5HA(57-76) epitope is unique for the H5N1 strain but conserved among all H5N1 clades recommended for vaccine development by World Health Organization. The unique H5HA(57-76) response was uncommon in unexposed individuals and only observed in the naïve T cell subset. Thus, H5N1 strain-specific H5HA(57-76) immunogenic epitope represents a unique marker for monitoring the efficacy of vaccination or as a candidate vaccine peptide. |
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| Affiliations: | Benaroya Research Institute at Virginia Mason, Seattle, WA 98101-2795, United States. |
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| Date: | 2009 |
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| Reference Type: | Literature |
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| PubMed ID: | 19446935 |
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| Journal: | Vaccine |
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| Journal Volume: | 27 |
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| Article Pages: | 3862-9 |
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| Journal ISSN: | 1873-2518 |
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| Article Chemical List: | Epitopes, T-Lymphocyte;HLA-DR4 Antigen;Hemagglutinin Glycoproteins, Influenza Virus;hemagglutinin, avian influenza A virus |
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| Article MeSH List: | Animals; CD4-Positive T-Lymphocytes(immunology); Cells, Cultured; Epitope Mapping; Epitopes, T-Lymphocyte(immunology); HLA-DR4 Antigen(immunology); Hemagglutinin Glycoproteins, Influenza Virus(immunology); Humans; Influenza A Virus, H5N1 Subtype(immunology); Mice; Mice, Transgenic |
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| Curation Last Updated: | 2011-03-28 23:46:57 |
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