Epitopes described in "A naturally processed epitope on rotavirus VP7 glycoprotein recognized by HLA-A2.1-restricted cytotoxic CD8+ T cells."

Article Authors:Jing Wei; Jintao Li; Xiaoping Zhang; Yan Tang; Jingxue Wang; Yuzhang Wu
Article Title:A naturally processed epitope on rotavirus VP7 glycoprotein recognized by HLA-A2.1-restricted cytotoxic CD8+ T cells.
Reference Detail
Reference ID:1015108
Abstract:Previous studies in mice have shown that CD8(+) T cells can mediate clearance of rotavirus (RV) infection and provide protection from reinfection. Moreover, it has been demonstrated that VP7 can induce a cross-reactive and vigorous specific cytotoxic T-lymphocyte (CTL) response. In this study, a panel of the potential human leukocyte antigen (HLA)-A2.1-restricted CTL epitopes derived from the human RV Wa strain VP7 glycoprotein were screened and assessed using reverse immunogenetics. The specific CTLs induced in vitro by p18-26 (LLNYILKSV)-pulsed autologous dendritic cells from the peripheral blood lymphocytes of healthy HLA-A2.1 donors released interferon-gamma (IFN-gamma) specifically upon stimulation of p18-26, and meanwhile these CTLs lysed p18-26-loaded T2 cells and human RV Wa strain-infected HLA-A2.1(+) Caco-2 cells in an HLA-A2.1-restricted manner. P18-26 was still proved to be immunogenic in vivo in HLA-A2.1/Kb transgenic mice. We propose that the newly identified CTL epitope restricted by HLA-A2.1 could aid in further study of RV-associated infection in humans.
Affiliations:Institute of Immunology, Third Military Medical University , District Shapingba, Chongqing, People's Republic of China. weijing06@yahoo.com.cn
Reference Type:Literature
PubMed ID:19435415
Journal:Viral Immunol
Journal Volume:22
Article Pages:189-94
Journal ISSN:1557-8976
Article Chemical List:Antigens, Viral;Capsid Proteins;Epitopes;HLA-A2 Antigen;VP7 protein, Rotavirus;Interferon-gamma
Article MeSH List:Animals; Antigen Presentation; Antigens, Viral(immunology; metabolism); CD8-Positive T-Lymphocytes(immunology); Capsid Proteins(immunology; metabolism); Cell Line; Epitopes(immunology; metabolism); HLA-A2 Antigen(immunology; metabolism); Humans; Interferon-gamma(secretion); Leukocytes, Mononuclear(immunology; secretion); Lymphocyte Activation; Mice; Rotavirus(immunology)
Curation Last Updated:2014-10-03 21:35:18