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| Article Authors: | Peter Klucar; Peter F Barnes; Ying Kong; Susan T Howard; Xiuhua Pang; Fang-Fang Huang; Amy R Tvinnereim; Buka Samten; Homayoun Shams |
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| Article Title: | Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis. |
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| Reference ID: | 1014040 |
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| Abstract: | To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1 0401, because CFP10 contains epitopes for DRB1 0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis |
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| Affiliations: | Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center at Tyler, Tyler, TX 75708, United States. |
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| Date: | 2009 |
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| Reference Type: | Literature |
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| PubMed ID: | 19402204 |
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| Journal: | Vaccine |
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| Journal Volume: | 27 |
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| Article Pages: | 1816-24 |
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| Journal ISSN: | 0264-410X |
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| Article Chemical List: | Antigens, Bacterial;CFP-10 protein (71-85), Mycobacterium tuberculosis;HLA-DR1 Antigen;Lamp1 protein, mouse;Lysosomal-Associated Membrane Protein 2;Lysosome-Associated Membrane Glycoproteins;Peptide Fragments;Vaccines, DNA;Vaccines, Synthetic;Interferon-gamma;Polyethyleneimine |
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| Article MeSH List: | Animals; Antigens, Bacterial(genetics; immunology); CD4-Positive T-Lymphocytes(immunology); HLA-DR1 Antigen(immunology); Immunization; Interferon-gamma(immunology); Lung(cytology; immunology); Lysosomal-Associated Membrane Protein 2(immunology); Lysosome-Associated Membrane Glycoproteins(immunology); Lysosomes(immunology); Mice; Mice, Inbred C57BL; Mycobacterium tuberculosis(immunology); Peptide Fragments(immunology); Plasmids(immunology); Polyethyleneimine(chemistry); Spleen(cytology; immunology); Tuberculosis, Pulmonary(immunology; prevention & control); Vaccines, DNA(immunology); Vaccines, Synthetic(immunology) |
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| Curation Last Updated: | 2013-05-28 21:38:25 |
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