Epitopes described in "Reevaluating the CD8 T-cell response to herpes simplex virus type 1: involvement of CD8 T cells reactive to subdominant epitopes."

Article Authors:Brian S Sheridan; Thomas L Cherpes; Julie Urban; Pawel Kalinski; Robert L Hendricks
Article Title:Reevaluating the CD8 T-cell response to herpes simplex virus type 1: involvement of CD8 T cells reactive to subdominant epitopes.
Reference Detail
Reference ID:1013669
Abstract:In C57BL/6 (B6) mice, most herpes simplex virus (HSV)-specific CD8 T cells recognize a strongly immunodominant epitope on glycoprotein B (gB498) and can inhibit HSV type 1 (HSV-1) reactivation from latency in trigeminal ganglia (TG). However, half of the CD8 T cells retained in latently infected TG of B6 mice are not gB498 specific and have been largely ignored. The following observations from our current study indicate that these gB498-nonspecific CD8 T cells are HSV specific and may contribute to the control of HSV-1 latency. First, following corneal infection, OVA257-specific OT-1 CD8 T cells do not infiltrate the infected TG unless mice are simultaneously immunized with OVA257 peptide, and then they are not retained. Second, 30% of CD8 T cells in acutely infected TG that produce gamma interferon in response to HSV-1 stimulation directly ex vivo are gB498 nonspecific, and these cells maintain an activation phenotype during viral latency. Finally, gB498-nonspecific CD8 T cells are expanded in ex vivo cultures of latently infected TG and inhibit HSV-1 reactivation from latency in the absence of gB498-specific CD8 T cells. We conclude that many of the CD8 T cells that infiltrate and are retained in infected TG are HSV specific and potentially contribute to maintenance of HSV-1 latency. Identification of the viral proteins recognized by these cells will contribute to a better understanding of the dynamics of HSV-1 latency.
Affiliations:Graduate Program in Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Reference Type:Literature
PubMed ID:19073721
Journal:J Virol
Journal Volume:83
Article Pages:2237-45
Journal ISSN:1098-5514
Article Chemical List:Antigens, Viral;Epitopes, T-Lymphocyte;Viral Envelope Proteins;glycoprotein B, Simplexvirus;Interferon-gamma
Article MeSH List:Animals; Antigens, Viral(immunology); CD8-Positive T-Lymphocytes(immunology); Cells, Cultured; Epitopes, T-Lymphocyte(immunology); Female; Herpes Simplex(immunology; virology); Herpesvirus 1, Human(immunology; physiology); Interferon-gamma(immunology); Lymphocyte Activation; Mice; Mice, Inbred C57BL; Trigeminal Ganglion(immunology; virology); Viral Envelope Proteins(immunology); Virus Latency
Curation Last Updated:2015-07-30 20:28:32