Epitopes described in "Parasite stage-specific recognition of endogenous Toxoplasma gondii-derived CD8+ T cell epitopes."

Article Authors:Eva-Maria Frickel; Nivedita Sahoo; Johnathan Hopp; Marc-Jan Gubbels; Mary Patricia J Craver; Laura J Knoll; Hidde L Ploegh; Gijsbert M Grotenbreg
Article Title:Parasite stage-specific recognition of endogenous Toxoplasma gondii-derived CD8+ T cell epitopes.
Reference Detail
Reference ID:1013710
Abstract:BACKGROUND: BALB/c mice control infection with the obligate intracellular parasite Toxoplasma gondii and develop a latent chronic infection in the brain, as do immunocompetent humans. Interferon-gamma-producing CD8+ T cells provide essential protection against T. gondii infection, but the epitopes recognized have so far remained elusive. METHODS: We employed caged major histocompatibility complex molecules to generate approximately 250 H-2L(d) tetramers and to distinguish T. gondii-specific CD8+ T cells in BALB/c mice. RESULTS: We identified 2 T. gondii-specific H-2L(d)-restricted T cell epitopes, one from dense granule protein GRA4 and the other from rhoptry protein ROP7. H-2L(d)/GRA4 reactive T cells from multiple organ sources predominated 2 weeks after infection, while the reactivity of the H-2L(d)/ROP7 T cells peaked 6-8 weeks after infection. BALB/c animals infected with T. gondii mutants defective in establishing a chronic infection showed altered levels of antigen-specific T cells, depending on the T. gondii mutant used. CONCLUSIONS: Our results shed light on the identity and the parasite stage-specificity of 2 CD8+ T cell epitopes recognized in the acute and chronic phase of infection with T. gondii.
Affiliations:Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA. frickel@wi.mit.edu
Reference Type:Literature
PubMed ID:18922097
Journal:J Infect Dis
Journal Volume:198
Article Pages:1625-33
Journal ISSN:0022-1899
Article Chemical List:Antibodies, Protozoan;Antigens, Protozoan;Epitopes, T-Lymphocyte;Protozoan Proteins
Article MeSH List:Animals; Antibodies, Protozoan; Antigens, Protozoan; CD8-Positive T-Lymphocytes(physiology); Epitopes, T-Lymphocyte(physiology); Genes, MHC Class I(immunology); Mice; Mice, Inbred BALB C; Protozoan Proteins(genetics); Toxoplasma(immunology); Toxoplasmosis(immunology)
Curation Last Updated:2015-07-30 20:28:36