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| Article Authors: | Haruhiko Murata; Belete Teferedegne; Li Sheng; Andrew M Lewis Jr; Keith Peden |
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| Article Title: | Identification of a neutralization epitope in the VP1 capsid protein of SV40. |
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| Reference ID: | 1013735 |
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| Abstract: | Three SV40 escape mutants were identified by selection in the presence of monoclonal antibodies with neutralizing activity. The VP1 amino acid alterations in these mutants were: (1) K73-->E (in loop BC); (2) D77-->E (in loop BC); (3) K171-->R (in loop EF); and (4) Q175-->H (in loop EF). These residues are clustered in close proximity to each other on the surface of the native capsid protein, strongly suggesting that they form a conformational epitope directly recognized by the neutralizing antibody. To our knowledge, the present study represents the first experimental mapping of a neutralization epitope of a polyomavirus family member. Structural information regarding the neutralization epitope should be useful for clarifying the extent of cross-reactivity exhibited by the humoral immune response towards related primate polyomaviruses (e.g., SV40, BKV, and JCV). |
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| Affiliations: | Laboratory of DNA Viruses, Division of Viral Products CBER, FDA Bethesda, MD 20892, USA. |
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| Date: | 2008 |
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| Reference Type: | Literature |
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| PubMed ID: | 18789470 |
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| Journal: | Virology |
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| Journal Volume: | 381 |
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| Article Pages: | 116-22 |
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| Journal ISSN: | 1096-0341 |
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| Article Chemical List: | Antibodies, Viral;Capsid Proteins;Epitopes |
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| Article MeSH List: | Antibodies, Viral(immunology; metabolism); Capsid Proteins(chemistry; genetics; immunology); Cell Line; Epitope Mapping; Epitopes(chemistry; immunology); Humans; Mutation(genetics); Neutralization Tests; Phenotype; Simian virus 40(genetics; immunology) |
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| Curation Last Updated: | 2011-09-30 20:03:06 |
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