Epitopes described in "Identification of a neutralization epitope in the VP1 capsid protein of SV40."

Article Authors:Haruhiko Murata; Belete Teferedegne; Li Sheng; Andrew M Lewis Jr; Keith Peden
Article Title:Identification of a neutralization epitope in the VP1 capsid protein of SV40.
Reference Detail
Reference ID:1013735
Abstract:Three SV40 escape mutants were identified by selection in the presence of monoclonal antibodies with neutralizing activity. The VP1 amino acid alterations in these mutants were: (1) K73-->E (in loop BC); (2) D77-->E (in loop BC); (3) K171-->R (in loop EF); and (4) Q175-->H (in loop EF). These residues are clustered in close proximity to each other on the surface of the native capsid protein, strongly suggesting that they form a conformational epitope directly recognized by the neutralizing antibody. To our knowledge, the present study represents the first experimental mapping of a neutralization epitope of a polyomavirus family member. Structural information regarding the neutralization epitope should be useful for clarifying the extent of cross-reactivity exhibited by the humoral immune response towards related primate polyomaviruses (e.g., SV40, BKV, and JCV).
Affiliations:Laboratory of DNA Viruses, Division of Viral Products CBER, FDA Bethesda, MD 20892, USA.
Reference Type:Literature
PubMed ID:18789470
Journal Volume:381
Article Pages:116-22
Journal ISSN:1096-0341
Article Chemical List:Antibodies, Viral;Capsid Proteins;Epitopes
Article MeSH List:Antibodies, Viral(immunology; metabolism); Capsid Proteins(chemistry; genetics; immunology); Cell Line; Epitope Mapping; Epitopes(chemistry; immunology); Humans; Mutation(genetics); Neutralization Tests; Phenotype; Simian virus 40(genetics; immunology)
Curation Last Updated:2014-10-03 21:21:37