Epitopes described in "Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen."

Article Authors:Peter Klucar; Peter F Barnes; Ying Kong; Buka Samten; Amy Tvinnereim; Ralf Spallek; Gerald T Nepom; Mahavir Singh; Homayoun Shams
Article Title:Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen.
Reference Detail
Reference ID:1013185
Abstract:CD4+ T cells are believed to play a dominant role in human defenses against Mycobacterium tuberculosis through production of interferon (IFN)-gamma, cytolytic T-cell (CTL) activity, and inhibition of intracellular mycobacterial growth. Most functional studies of CD4+ cells have used bulk T-cells that recognize crude mycobacterial antigens, and the functional capacity of individual human T cells is not well defined. We studied the functional capacity of human CD4+ T-cell clones that recognize a specific mycobacterial peptide. Clone B9 produced high concentrations of IFN-gamma and exhibited potent CTL activity, whereas clone D3 produced IFN-gamma but showed poor CTL activity. The CTL activity of clone B9 was inhibited by SrCl(2) and concanamycin A but not by anti-Fas antibodies. Clone B9 also reduced the mycobacterial burden in dendritic cells by more than 90%, and this antimycobacterial activity was inhibited by SrCl(2) and concanamycin A. We conclude that: (1) individual human peptide-specific CD4+ T-cell clones have differential capacity to produce Th1 cytokines and to lyse M tuberculosis-infected target cells; and (2) both granulysin and perforin contribute to the capacity of human CD4+ T-cells to lyse infected targets and to inhibit intracellular mycobacterial growth.
Affiliations:Department of Microbiology and Immunology, University of Texas Health Center at Tyler, Tyler, TX, USA.
Reference Type:Literature
PubMed ID:18588932
Journal:Hum Immunol
Journal Volume:69
Article Pages:475-83
Journal ISSN:0198-8859
Article Chemical List:Antigens, Differentiation, T-Lymphocyte;Bacterial Proteins;Cytokines;GNLY protein, human;Macrolides;Peptide Fragments;Perforin;concanamycin A;Interferon-gamma
Article MeSH List:Antigens, Differentiation, T-Lymphocyte(immunology); Bacterial Proteins(immunology); CD4-Positive T-Lymphocytes(immunology; metabolism); Clone Cells; Cytokines(biosynthesis; immunology); Cytotoxicity, Immunologic; Dendritic Cells(microbiology); Humans; Interferon-gamma(biosynthesis; immunology); Macrolides(pharmacology); Mycobacterium tuberculosis(growth & development; immunology); Peptide Fragments(immunology); Perforin(physiology)
Curation Last Updated:2014-10-03 21:13:40