Epitopes described in "Significantly improved accuracy of diagnosis of early Lyme disease by peptide enzyme-linked immunosorbent assay based on the borreliacidal antibody epitope of Borrelia burgdorferi OspC."

Reference
Article Authors:Dean A Jobe; Steven D Lovrich; Krista E Asp; Michelle A Mathiason; Stephanie E Albrecht; Ronald F Schell; Steven M Callister
Article Title:Significantly improved accuracy of diagnosis of early Lyme disease by peptide enzyme-linked immunosorbent assay based on the borreliacidal antibody epitope of Borrelia burgdorferi OspC.
Reference Detail
Reference ID:1012358
Abstract:Highly specific borreliacidal antibodies are induced by infection with Borrelia burgdorferi, and the immunodominant response during early Lyme disease is specific for an epitope within the 7 amino acids nearest the C terminus of OspC. We evaluated the ability of an enzyme-linked immunosorbent assay (ELISA) based on a synthetic peptide (OspC7) that matched the region to detect the response and compared the sensitivity during early Lyme disease to that for an FDA-approved Western blot. When the optical density value was adjusted to 98% specificity based on the results from testing normal or uncharacterized sera (n = 236) or sera from patients with blood factors or illnesses that commonly produce antibodies that cross-react with B. burgdorferi antigens (n = 77), 115 (73%) of 157 sera from patients likely to have early Lyme disease were positive for immunoglobulin M (IgM) antibodies and 17 (11%) also had IgG antibodies. In addition, the IgM ELISA reactivities and the titers of antibodies detected by a flow cytometric borreliacidal antibody test correlated closely (r = 0.646). Moreover, the IgM ELISA was significantly more sensitive (P < 0.001) than the Western blot procedure. The findings therefore confirmed that the peptide IgM ELISA detected OspC borreliacidal antibodies and provided strong evidence that the test can eliminate the necessity for confirming early Lyme disease by a supplementary test such as Western blotting.
Date:2008
Reference Type:Literature
PubMed ID:18417666
Journal:Clin Vaccine Immunol
Journal Volume:15
Article Pages:981-5
Journal ISSN:1556-6811
Article Chemical List:Antibodies, Bacterial;Antigens, Bacterial;Bacterial Outer Membrane Proteins;Epitopes;Immunoglobulin G;Immunoglobulin M;OspC protein
Article MeSH List:Antibodies, Bacterial(blood; immunology); Antigens, Bacterial(blood; immunology); Bacterial Outer Membrane Proteins(blood; immunology); Borrelia burgdorferi(immunology); Enzyme-Linked Immunosorbent Assay(methods); Epitopes(immunology; metabolism); Humans; Immunoglobulin G(blood); Immunoglobulin M(blood); Lyme Disease(diagnosis; immunology; microbiology); Sensitivity and Specificity
Curation Last Updated:2014-10-03 21:10:30