Epitopes described in "Glycolipid studies in small intestine and pancreas of alpha1,3-galactosyltransferase knockout miniature swine: alpha1,3GALT-KO animals lack alphaGAL antigens and contain novel blood group H compounds."

Reference
Article Authors:M Diswall; J Angström; H-J Schuurman; F J M F Dor; L Rydberg; M E Breimer
Article Title:Glycolipid studies in small intestine and pancreas of alpha1,3-galactosyltransferase knockout miniature swine: alpha1,3GALT-KO animals lack alphaGAL antigens and contain novel blood group H compounds.
Reference Detail
Reference ID:1020544
Abstract:BACKGROUND: To avoid hyperacute rejection of xeno-organs, alpha1,3-galactosyltransferase knock-out (GalT-KO) pigs have been produced. However, Galalpha1,3Gal (Gal) determinant elimination may expose cryptic carbohydrate antigens and/or generate new antigens that might interfere with the human immune response. METHODS: Glycolipids isolated from small intestine and pancreas of two GalT-KO and one wild-type (WT) pig were tested for immune reactivity with antibodies on thin-layer chromatograms after separation by high-performance liquid chromatography, and selected fractions were analysed by proton NMR spectroscopy. RESULTS: Immunostaining using purified human anti-Gal Abs revealed that tissues from WT animals express large amounts of Gal-antigens whereas GalT-KO tissues lacked these antigens. Proton NMR spectroscopy on small intestine fractions revealed both linear and branched nona- and decaglycosylceramides, respectively, with terminal Gal-epitopes. In corresponding GalT-KO fractions, Gal-epitopes seemed to be replaced by terminal alpha1,2fucoses. Two novel branched blood group H compounds was found in the GalT-KO intestine. CONCLUSIONS: The structural complexity of alphaGal-terminating antigens in the WT organs is very high. Knockout of alpha1,3GalT by gene-targeting results in elimination of Gal-determinants. In addition structurally novel alpha1,2fucose-terminated blood group H compounds were identified in the GalT-KO tissue. These compounds are not expected to be recognized by the human immune system.
Date:2008
Reference Type:Literature
PubMed ID:18374124
Journal:Transplant Proc
Journal Volume:40
Article Pages:543-6
Journal ISSN:0041-1345
Article Chemical List:ABO Blood-Group System;Antigens;Glycolipids;Galactosyltransferases;N-acetyllactosaminide alpha-1,3-galactosyltransferase;Galactose
Article MeSH List:ABO Blood-Group System(genetics); Animals; Antigens(genetics); Galactose(genetics); Galactosyltransferases(deficiency; genetics); Glycolipids(metabolism); Humans; Intestine, Small(enzymology; metabolism); Organisms, Genetically Modified; Pancreas(metabolism); Swine(genetics); Swine, Miniature(genetics); Transplantation, Heterologous
Curation Last Updated:2014-09-11 20:11:15