Epitopes described in "Identification and in vitro expansion of functional antigen-specific CD25+ FoxP3+ regulatory T cells in hepatitis C virus infection."

Article Authors:Hirotoshi Ebinuma; Nobuhiro Nakamoto; Yun Li; David A Price; Emma Gostick; Bruce L Levine; J Tobias; William W Kwok; Kyong-Mi Chang
Article Title:Identification and in vitro expansion of functional antigen-specific CD25+ FoxP3+ regulatory T cells in hepatitis C virus infection.
Reference Detail
Reference ID:1012382
Abstract:CD4(+)CD25(+) regulatory T cells (CD25(+) Tregs) play a key role in immune regulation. Since hepatitis C virus (HCV) persists with increased circulating CD4(+)CD25(+) T cells and virus-specific effector T-cell dysfunction, we asked if CD4(+)CD25(+) T cells in HCV-infected individuals are similar to natural Tregs in uninfected individuals and if they include HCV-specific Tregs using the specific Treg marker FoxP3 at the single-cell level. We report that HCV-infected patients display increased circulating FoxP3(+) Tregs that are phenotypically and functionally indistinguishable from FoxP3(+) Tregs in uninfected subjects. Furthermore, HCV-specific FoxP3(+) Tregs were detected in HCV-seropositive persons with antigen-specific expansion, major histocompatibility complex class II/peptide tetramer binding affinity, and preferential suppression of HCV-specific CD8 T cells. Transforming growth factor beta contributed to antigen-specific Treg expansion in vitro, suggesting that it may contribute to antigen-specific Treg expansion in vivo. Interestingly, FoxP3 expression was also detected in influenza virus-specific CD4 T cells. In conclusion, functionally active and virus-specific FoxP3(+) Tregs are induced in HCV infection, thus providing targeted immune regulation in vivo. Detection of FoxP3 expression in non-HCV-specific CD4 T cells suggests that immune regulation through antigen-specific Treg induction extends beyond HCV.
Affiliations:Philadelphia Veterans Affairs Medical Center, Philadelphia, PA 19104, USA.
Reference Type:Literature
PubMed ID:18337568
Journal:J Virol
Journal Volume:82
Article Pages:5043-53
Journal ISSN:1098-5514
Article Chemical List:Antigens, CD4;FOXP3 protein, human;Forkhead Transcription Factors;Histocompatibility Antigens Class II;Interleukin-2 Receptor alpha Subunit;Transforming Growth Factor beta
Article MeSH List:Antigens, CD4(analysis); CD8-Positive T-Lymphocytes(immunology); Cell Proliferation; Cells, Cultured; Flow Cytometry; Forkhead Transcription Factors(analysis); Hepacivirus(immunology); Hepatitis C(immunology); Histocompatibility Antigens Class II(metabolism); Humans; Interleukin-2 Receptor alpha Subunit(analysis); Protein Binding; T-Lymphocyte Subsets(immunology); T-Lymphocytes, Regulatory(chemistry; immunology); Transforming Growth Factor beta(metabolism)
Curation Last Updated:2015-07-24 20:08:52