Epitopes described in "Vaccine-elicited 10-kilodalton culture filtrate protein-specific CD8+ T cells are sufficient to mediate protection against Mycobacterium tuberculosis infection."

Article Authors:Ying Wu; Joshua S Woodworth; Daniel S Shin; Sheldon Morris; Samuel M Behar
Article Title:Vaccine-elicited 10-kilodalton culture filtrate protein-specific CD8+ T cells are sufficient to mediate protection against Mycobacterium tuberculosis infection.
Reference Detail
Reference ID:1012384
Abstract:The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Mycobacterium bovis bacillus Calmette-Guérin (BCG), and it is hypothesized that these proteins are important targets of protective immunity. Indeed, vaccination with ESAT-6 elicits protective CD4+ T cells in C57BL/6 mice. We have previously shown that M. tuberculosis infection of C3H mice elicits CFP-10-specific CD8+ and CD4+ T cells. Here we demonstrate that immunization with a CFP-10 DNA vaccine stimulates a specific T-cell response only to the H-2K(k)-restricted epitope CFP-10(32-39). These CFP-10(32-39)-specific CD8+ cells undergo a rapid expansion and accumulate in the lung following challenge of immunized mice with aerosolized M. tuberculosis. Protective immunity is induced by CFP-10 DNA vaccination as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis. These data demonstrate that CFP-10 is a protective antigen and that CFP-10(32-39)-specific CD8+ T cells elicited by vaccination are sufficient to mediate protection against tuberculosis.
Affiliations:Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Smith Building Room 516C, One Jimmy Fund Way, Boston, MA 02115, USA.
Reference Type:Literature
PubMed ID:18332205
Journal:Infect Immun
Journal Volume:76
Article Pages:2249-55
Journal ISSN:0019-9567
Article Chemical List:Bacterial Proteins;CFP-10 protein, Mycobacterium tuberculosis;Epitopes, T-Lymphocyte;Tuberculosis Vaccines;Vaccines, DNA;Interferon-gamma
Article MeSH List:Animals; Bacterial Proteins(genetics; immunology); CD8-Positive T-Lymphocytes(immunology); Colony Count, Microbial; Epitopes, T-Lymphocyte(immunology); Female; Interferon-gamma(biosynthesis); Lung(immunology; microbiology); Lymph Nodes(immunology); Mice; Mice, Inbred C3H; Mycobacterium Infections(prevention & control); Mycobacterium tuberculosis(immunology; isolation & purification); Spleen(immunology; microbiology); Tuberculosis Vaccines(genetics; immunology); Vaccines, DNA(genetics; immunology)
Curation Last Updated:2015-07-30 20:24:11