Epitopes described in "Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy."

Article Authors:Annebeth E Flinterman; Edward F Knol; Doerthe A Lencer; Ludmilla Bardina; Constance F den Hartog Jager; Jing Lin; Suzanne G M A Pasmans; Carla A F M Bruijnzeel-Koomen; Hugh A Sampson; Els van Hoffen; Wayne G Shreffler
Article Title:Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy.
Reference Detail
Reference ID:1013996
Abstract:BACKGROUND: Better understanding of the relationship between antibody response to peanut and clinical sensitivity might lead to more accurate prognostication. OBJECTIVE: We sought to investigate peanut-specific IgE and IgG4 epitope diversity in relation to challenge-defined clinical sensitivity to peanut in a group of peanut-sensitized children. METHODS: Clinical sensitivity was determined by means of double-blind, placebo-controlled peanut challenges in 24 sensitized children. Six atopic control subjects were included. Specific IgE and IgG4 binding to 419 overlapping 15-amino-acid peptides representing the sequence of recombinant Ara h 1, Ara h 2, and Ara h3 was analyzed by means of microarray immunoassay. RESULTS: Peanut-sensitized patient sera bound significantly more IgE and IgG4 epitopes than control sera. This patient group reacted to the same Ara h 1, Ara h 2, and Ara h 3 epitopes as reported previously. There was a positive correlation between IgE epitope diversity (ie, number of epitopes recognized) and clinical sensitivity (r = 0.6), such that patients with the greatest epitope diversity were significantly more sensitive than those with the lowest diversity (P = .021). No specific epitopes were associated with severe reactions to peanut. IgG4 binding was observed to largely similar epitopes but was less pronounced than IgE binding and did not relate to the clinical sensitivity to peanut. IgE and IgG4 epitope-recognition patterns were largely stable over a 20-month period. CONCLUSION: Clinical sensitivity, as determined by means of double-blind, placebo-controlled peanut challenge, is positively related to a more polyclonal IgE response, which remains stable over time.
Affiliations:Department of Dermatology/Allergology, University Medical Centre Utrecht, Utrecht, The Netherlands.
Reference Type:Literature
PubMed ID:18234310
Journal:J Allergy Clin Immunol
Journal Volume:121
Article Pages:737-743.e10
Journal ISSN:1097-6825
Article Chemical List:Allergens;Epitopes, B-Lymphocyte;Immunoglobulin G;Immunoglobulin E
Article MeSH List:Adolescent; Allergens(immunology); Antibody Specificity; Arachis hypogaea(chemistry; immunology); Child; Child, Preschool; Epitopes, B-Lymphocyte(chemistry; immunology); Humans; Immunoglobulin E(blood; chemistry; immunology); Immunoglobulin G(blood; chemistry; immunology); Infant; Male; Peanut Hypersensitivity(blood; immunology)
Curation Last Updated:2014-10-03 21:23:59