Epitopes described in "Differential role of gamma interferon in inhibiting pulmonary eosinophilia and exacerbating systemic disease in fusion protein-immunized mice undergoing challenge infection with respiratory syncytial virus."

Reference
Article Authors:Elaine M Castilow; Matthew R Olson; David K Meyerholz; Steven M Varga
Article Title:Differential role of gamma interferon in inhibiting pulmonary eosinophilia and exacerbating systemic disease in fusion protein-immunized mice undergoing challenge infection with respiratory syncytial virus.
Reference Detail
Reference ID:1007942
Abstract:Secondary exposure to respiratory syncytial virus (RSV) can lead to immunopathology and enhanced disease in vaccinated individuals. Vaccination with individual RSV proteins influences the type of secondary RSV-specific immune response that develops upon challenge RSV infection, as well as the extent of immunopathology. RSV-specific memory CD4 T cells can directly contribute to immunopathology through their cytokine production. Immunization of BALB/c mice with a recombinant vaccinia virus (vv) expressing the attachment (G) protein of RSV results in pulmonary eosinophilia upon RSV challenge, whereas immunization of mice with a vv expressing the fusion (F) protein does not. We analyzed the CD4 T-cell response to an I-E(d)-restricted CD4 T-cell epitope within the F protein of RSV corresponding to amino acids 51 to 66 in an effort to better understand the similarities and differences in the immune response elicited by the G versus the F protein. Vaccination with the G protein induces a mixture of RSV G-specific Th1 and Th2 cells with a restricted T-cell receptor repertoire. In contrast, we demonstrate here that immunization with the F protein elicits a broad repertoire of RSV F-specific CD4 T cells that predominantly exhibit a Th1 phenotype. However, in the absence of gamma interferon (IFN-gamma), RSV F(51-66)-specific CD4 T cells secreted interleukin-5, and mice developed pulmonary eosinophilia after RSV challenge. IFN-gamma-deficient mice exhibited decreased weight loss compared to wild-type controls, suggesting that IFN-gamma exacerbates systemic disease. These data demonstrate that IFN-gamma can have both beneficial and detrimental effects during a secondary RSV infection.
Date:2008
Reference Type:Literature
PubMed ID:18094193
Journal:J Virol
Journal Volume:82
Article Pages:2196-207
Journal ISSN:1098-5514
Article Chemical List:Recombinant Fusion Proteins;Viral Vaccines;Interferon-gamma
Article MeSH List:Amino Acid Sequence; Animals; Bronchoalveolar Lavage Fluid(virology); CD4-Positive T-Lymphocytes(immunology); Enzyme-Linked Immunosorbent Assay; Female; Immunologic Memory; Interferon-gamma(physiology); Lung(virology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Pulmonary Eosinophilia(pathology; prevention & control); Recombinant Fusion Proteins(administration & dosage); Respiratory Syncytial Virus Infections(immunology; prevention & control); Th1 Cells(immunology); Th2 Cells(immunology); Viral Vaccines(administration & dosage; immunology)
Curation Last Updated:2014-10-03 20:59:35