Epitopes described in "The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein."

Article Authors:David Onion; Laura J Crompton; Donald W Milligan; Paul A H Moss; Steven P Lee; Vivien Mautner
Article Title:The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein.
Reference Detail
Reference ID:1012052
Abstract:Adenovirus is a significant pathogen in immunocompromised patients and is widely utilized as a gene delivery vector, so a detailed understanding of the human immune response to adenovirus infection is critical. This study characterized the adenovirus-specific CD4(+) T-cell response of healthy donors by incubation with whole virus or with individual hexon and fiber proteins. Adenovirus-specific CD4(+) T cells averaged 0.26 % of the CD4(+) T-cell pool and were detectable in all donors. T cells recognizing the highly conserved hexon protein accounted for 0.09 %, whereas no response was observed against the fiber protein. A panel of hexon-specific CD4(+) T-cell clones was generated and shown to lyse targets infected with adenovirus from different serotypes and species. Three CD4 T-cell epitopes are described, which map to highly conserved regions of the hexon protein.
Affiliations:CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK. d.f.onion@bham.ac.uk
Reference Type:Literature
PubMed ID:17698650
Journal:J Gen Virol
Journal Volume:88
Article Pages:2417-25
Journal ISSN:0022-1317
Article Chemical List:Capsid Proteins;HLA Antigens;hexon capsid protein, Adenovirus
Article MeSH List:CD4-Positive T-Lymphocytes(immunology; virology ); CD8-Positive T-Lymphocytes(immunology ); Capsid Proteins(chemistry; immunology ); Cell Line; Conserved Sequence; HLA Antigens(immunology ); Humans; Lymphocyte Depletion; T-Lymphocytes(immunology )
Curation Last Updated:2014-10-03 21:09:16