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| Article Authors: | H Martin Vordermeier; Jemma Brown; Paul J Cockle; Willeke P J Franken; Jan Wouter Drijfhout; Sandra M Arend; Tom H M Ottenhoff; Keith Jahans; R Glyn Hewinson |
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| Article Title: | Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level. |
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| Reference ID: | 1005996 |
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| Abstract: | Cross-reactivity between Mycobacterium kansasii ESAT-6 and CFP-10 homologues and their M. bovis counterparts can confound the interpretation of immunodiagnostic tests for tuberculosis. M. kansasii is a nontuberculous mycobacterial species cultured from skin test-positive cattle in Great Britain. Using peptides derived from M. bovis and M. kansasii ESAT-6 and CFP-10 regions that differ between these species, we investigated the species specificity and cross-reactivity at the level of individual bovine T-cell epitopes. Our results demonstrated that all peptides tested are fully cross-reactive, with the exception of one ESAT-6-derived peptide that harbored an M. bovis-specific epitope(s) when it was recognized in the context of bovine leukocyte antigen (BoLA)-DQ but that was cross-reactive with its M. kansasii homologues when it was restricted by BoLA-DR. This observation further highlights that prediction of species specificity by comparing sequence identity/homology alone is not sufficient and that individuals with diverse major histocompatibility complex constellations need to be tested to characterize the cross-reactivity or species specificity of peptide-based reagents. |
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| Affiliations: | TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone KT15 3NB, United Kingdom. m.vordermeier@vla.defra.gsi.gov.uk |
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| Date: | 2007 |
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| Reference Type: | Literature |
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| PubMed ID: | 17671227 |
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| Journal: | Clin Vaccine Immunol |
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| Journal Volume: | 14 |
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| Article Pages: | 1203-9 |
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| Journal ISSN: | 1556-6811 |
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| Article Chemical List: | Antigens, Bacterial;Bacterial Proteins;CFP-10 protein, Mycobacterium tuberculosis;ESAT-6 protein, Mycobacterium tuberculosis;Epitopes, T-Lymphocyte |
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| Article MeSH List: | Amino Acid Sequence; Animals; Antigens, Bacterial(immunology); Bacterial Proteins(immunology); Birds; Cattle; Cross Reactions(immunology); Epitopes, T-Lymphocyte(immunology); Molecular Sequence Data; Mycobacterium Infections, Atypical(diagnosis; immunology); Mycobacterium bovis(immunology; isolation & purification); Mycobacterium kansasii(immunology; isolation & purification); Species Specificity |
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| Article Comments: | ErratumIn(Clin Vaccine Immunol. 2007 Nov;14(11):1536 Drijfhout, Jan Wouter [added] ) |
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| Curation Last Updated: | 2013-05-28 20:56:12 |
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