Epitopes described in "Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level."

Article Authors:H Martin Vordermeier; Jemma Brown; Paul J Cockle; Willeke P J Franken; Jan Wouter Drijfhout; Sandra M Arend; Tom H M Ottenhoff; Keith Jahans; R Glyn Hewinson
Article Title:Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level.
Reference Detail
Reference ID:1005996
Abstract:Cross-reactivity between Mycobacterium kansasii ESAT-6 and CFP-10 homologues and their M. bovis counterparts can confound the interpretation of immunodiagnostic tests for tuberculosis. M. kansasii is a nontuberculous mycobacterial species cultured from skin test-positive cattle in Great Britain. Using peptides derived from M. bovis and M. kansasii ESAT-6 and CFP-10 regions that differ between these species, we investigated the species specificity and cross-reactivity at the level of individual bovine T-cell epitopes. Our results demonstrated that all peptides tested are fully cross-reactive, with the exception of one ESAT-6-derived peptide that harbored an M. bovis-specific epitope(s) when it was recognized in the context of bovine leukocyte antigen (BoLA)-DQ but that was cross-reactive with its M. kansasii homologues when it was restricted by BoLA-DR. This observation further highlights that prediction of species specificity by comparing sequence identity/homology alone is not sufficient and that individuals with diverse major histocompatibility complex constellations need to be tested to characterize the cross-reactivity or species specificity of peptide-based reagents.
Affiliations:TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone KT15 3NB, United Kingdom. m.vordermeier@vla.defra.gsi.gov.uk.
Reference Type:Literature
PubMed ID:17671227
Journal:Clin Vaccine Immunol
Journal Volume:14
Article Pages:1203-9
Journal ISSN:1556-6811
Article Chemical List:Antigens, Bacterial;Bacterial Proteins;CFP-10 protein, Mycobacterium tuberculosis;ESAT-6 protein, Mycobacterium tuberculosis;Epitopes, T-Lymphocyte
Article MeSH List:Amino Acid Sequence; Animals; Antigens, Bacterial(immunology); Bacterial Proteins(immunology); Birds; Cattle; Cross Reactions(immunology); Epitopes, T-Lymphocyte(immunology); Molecular Sequence Data; Mycobacterium Infections, Nontuberculous(diagnosis; immunology); Mycobacterium bovis(immunology; isolation & purification); Mycobacterium kansasii(immunology; isolation & purification); Species Specificity
Article Comments:ErratumIn(Clin Vaccine Immunol. 2007 Nov;14(11):1536 Drijfhout, Jan Wouter [added] )
Curation Last Updated:2015-06-05 01:00:24