Epitopes described in "Mapping and characterization of the primary and anamnestic H-2(d)-restricted cytotoxic T-lymphocyte response in mice against human metapneumovirus."

Reference
Article Authors:Guillermina A Melendi; Fidel Zavala; Ursula J Buchholz; Guy Boivin; Peter L Collins; Steven R Kleeberger; Fernando P Polack
Article Title:Mapping and characterization of the primary and anamnestic H-2(d)-restricted cytotoxic T-lymphocyte response in mice against human metapneumovirus.
Reference Detail
Reference ID:1007958
Abstract:Cytotoxic T lymphocytes (CTLs) are important for the control of virus replication during respiratory infections. For human metapneumovirus (hMPV), an H-2(d)-restricted CTL epitope in the M2-2 protein has been described. In this study, we screened the hMPV F, G, N, M, M2-1, and M2-2 proteins using three independent algorithms to predict H-2(d) CTL epitopes in BALB/c mice. A dominant epitope (GYIDDNQSI) in positions 81 to 89 of the antitermination factor M2-1 and a subdominant epitope (SPKAGLLSL) in N(307-315) were detected during the anti-hMPV CTL response. Passive transfer of CD8(+) T-cell lines against M2-1(81-89) and N(307-315) protected Rag1(-/-) mice against hMPV challenge. Interestingly, diversification of CTL targets to include multiple epitopes was observed after repetitive infections. A subdominant response against the previously described M2-2 epitope was detected after the third infection. An understanding of the CTL response against hMPV is important for developing preventive and therapeutic strategies against the virus.
Date:2007
Reference Type:Literature
PubMed ID:17670840
Journal:J Virol
Journal Volume:81
Article Pages:11461-7
Journal ISSN:1098-5514
Article Chemical List:Epitopes;H-2 Antigens
Article MeSH List:Adoptive Transfer; Amino Acid Sequence; Animals; Epitope Mapping; Epitopes; H-2 Antigens(immunology); Humans; Metapneumovirus(immunology); Mice; Mice, Inbred BALB C; T-Lymphocytes, Cytotoxic(immunology; virology)
Curation Last Updated:2014-04-01 18:58:23