Epitopes described in "A fusion protein consisting of IL-16 and the encephalitogenic peptide of myelin basic protein constitutes an antigen-specific tolerogenic vaccine that inhibits experimental autoimmune encephalomyelitis."

Reference
Article Authors:Mark D Mannie; Derek J Abbott
Article Title:A fusion protein consisting of IL-16 and the encephalitogenic peptide of myelin basic protein constitutes an antigen-specific tolerogenic vaccine that inhibits experimental autoimmune encephalomyelitis.
Reference Detail
Reference ID:1015285
Abstract:To test a novel concept for the generation of tolerogenic vaccines, fusion proteins were constructed encompassing a tolerogenic or biasing cytokine and the major encephalitogenic peptide of guinea pig myelin basic protein (GPMBP; i.e., neuroantigen or NAg). The cytokine domain was predicted to condition APC while simultaneously targeting the covalently linked encephalitogenic peptide to the MHC class II Ag processing pathway of those conditioned APC. Rats were given three s.c. injections of cytokine-NAg in saline 1-2 wk apart and then at least 1 wk later were challenged with NAg in CFA. The rank order of tolerogenic activity in the Lewis rat model of EAE was NAgIL16 > IL2NAg > IL1RA-NAg, IL13NAg >or= IL10NAg, GPMBP, GP69-88, and saline. NAgIL16 was also an effective inhibitor of experimental autoimmune encephalomyelitis when administered after an encephalitogenic challenge during the onset of clinical signs. Covalent linkage of the NAg and IL-16 was required for inhibition of experimental autoimmune encephalomyelitis. These data identify IL-16 as an optimal cytokine partner for the generation of tolerogenic vaccines and indicate that such vaccines may serve as Ag-specific tolerogens for the treatment of autoimmune disease.
Affiliations:Department of Microbiology and Immunology, The Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA. manniem@ecu.edu
Date:2007
Reference Type:Literature
PubMed ID:17641011
Journal:J Immunol
Journal Volume:179
Article Pages:1458-65
Journal ISSN:0022-1767
Article Chemical List:Epitopes;Interleukin-16;Myelin Basic Protein;Peptide Fragments;Recombinant Fusion Proteins;Vaccines, DNA;myelin basic protein 68-88
Article MeSH List:Amino Acid Sequence; Animals; Cell Line; Encephalomyelitis, Autoimmune, Experimental(genetics; immunology; prevention & control); Epitopes(administration & dosage; genetics; immunology); Guinea Pigs; Immune Tolerance(genetics); Interleukin-16(administration & dosage; genetics; immunology); Molecular Sequence Data; Myelin Basic Protein(administration & dosage; genetics; immunology); Peptide Fragments(administration & dosage; genetics; immunology); Rats; Rats, Inbred Lew; Recombinant Fusion Proteins(administration & dosage; genetics; immunology); Vaccines, DNA(administration & dosage; genetics; immunology)
Curation Last Updated:2014-07-16 22:09:31