Epitopes described in "Matrix protein 2 vaccination and protection against influenza viruses, including subtype H5N1."

Article Authors:Stephen Mark Tompkins; Zi-Shan Zhao; Chia-Yun Lo; Julia A Misplon; Teresa Liu; Zhiping Ye; Robert J Hogan; Zhengqi Wu; Kimberly A Benton; Terrence M Tumpey; Suzanne L Epstein
Article Title:Matrix protein 2 vaccination and protection against influenza viruses, including subtype H5N1.
Reference Detail
Reference ID:1006150
Abstract:Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that crossreacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.
Reference Type:Literature
PubMed ID:17552096
Journal:Emerg Infect Dis
Journal Volume:13
Article Pages:426-35
Journal ISSN:1080-6059
Article Chemical List:Antibodies, Viral;Influenza Vaccines;Ion Channels;M2 protein, Influenza A virus;Recombinant Proteins;Vaccines, DNA;Vaccines, Synthetic;Viral Matrix Proteins
Article MeSH List:Adenoviridae(metabolism); Amino Acid Sequence; Animals; Antibodies, Viral(blood; immunology); Cross Reactions(immunology); Drug Evaluation, Preclinical; Female; Genes, Viral; Genetic Vectors(administration & dosage; metabolism); Immunization Schedule; Influenza A Virus, H5N1 Subtype(immunology); Influenza Vaccines(administration & dosage; immunology); Injections, Intramuscular; Injections, Intraperitoneal; Ion Channels(immunology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Orthomyxoviridae Infections(blood; prevention & control); Recombinant Proteins(biosynthesis; immunology); Sequence Alignment; T-Lymphocytes(immunology); Vaccination; Vaccines, DNA(immunology); Vaccines, Synthetic(genetics; immunology); Viral Matrix Proteins(genetics; immunology; metabolism)
Curation Last Updated:2014-10-03 20:45:27