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| Article Authors: | Stephen Mark Tompkins; Zi-Shan Zhao; Chia-Yun Lo; Julia A Misplon; Teresa Liu; Zhiping Ye; Robert J Hogan; Zhengqi Wu; Kimberly A Benton; Terrence M Tumpey; Suzanne L Epstein |
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| Article Title: | Matrix protein 2 vaccination and protection against influenza viruses, including subtype H5N1. |
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| Reference ID: | 1006150 |
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| Abstract: | Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that crossreacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A. |
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| Affiliations: | Food and Drug Administration, Bethesda, Maryland, USA. tompkins@vet.uga.edu |
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| Date: | 2007 |
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| Reference Type: | Literature |
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| PubMed ID: | 17552096 |
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| Journal: | Emerg Infect Dis |
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| Journal Volume: | 13 |
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| Article Pages: | 426-35 |
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| Journal ISSN: | 1080-6059 |
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| Article Chemical List: | Antibodies, Viral;Influenza Vaccines;Ion Channels;M2 protein, Influenza A virus;Recombinant Proteins;Vaccines, DNA;Vaccines, Synthetic;Viral Matrix Proteins |
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| Article MeSH List: | Adenoviridae(metabolism); Amino Acid Sequence; Animals; Antibodies, Viral(blood; immunology); Cross Reactions(immunology); Drug Evaluation, Preclinical; Female; Genes, Viral; Genetic Vectors(administration & dosage; metabolism); Immunization Schedule; Influenza A Virus, H5N1 Subtype(immunology); Influenza Vaccines(administration & dosage; immunology); Injections, Intramuscular; Injections, Intraperitoneal; Ion Channels(immunology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Orthomyxoviridae Infections(blood; prevention & control); Recombinant Proteins(biosynthesis; immunology); Sequence Alignment; T-Lymphocytes(immunology); Vaccination; Vaccines, DNA(immunology); Vaccines, Synthetic(genetics; immunology); Viral Matrix Proteins(genetics; immunology; metabolism) |
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| Curation Last Updated: | 2012-05-31 20:18:07 |
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