Epitopes described in "Epitope mapping of HSV-1 glycoprotein K (gK) reveals a T cell epitope located within the signal domain of gK."

Article Authors:Yanira Osorio; Kevin R Mott; Abdul M Jabbar; Alberto Moreno; Timothy P Foster; Konstantin G Kousoulas; Homayon Ghiasi
Article Title:Epitope mapping of HSV-1 glycoprotein K (gK) reveals a T cell epitope located within the signal domain of gK.
Reference Detail
Reference ID:1005978
Abstract:Glycoprotein K (gK) is a virion envelope component of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), which plays an important role in virion morphogenesis and egress. We previously demonstrated that immunization of mice with gK, but not with any of the 10 other HSV-1 glycoproteins, resulted in exacerbation of corneal scarring and herpetic dermatitis following ocular HSV-1 infection. However, little is known about the gK epitope(s) that is (are) involved in T cell activities in vitro or in vivo. Thus, epitope mapping of gK was performed using a panel of 15-mer peptides with five-amino acid overlaps spanning the full-length gK, and four expressed gK recombinant proteins representing different regions of gK. Epitope mapping within the gK polypeptide defined the amino acid sequence STVVLITAYGLVLVW as the predominant CD4(+) and CD8(+) T cell stimulatory region both in vitro and in vivo. IFN-gamma expression by CD4(+) T cells was CD8(+) T cells-dependent. This immunodominant epitope is located within the signal sequence of the gK polypeptide and is highly conserved in HSV-1 and HSV-2 strains. Using prediction algorithms, the peptide is predicted to bind to numerous MHC class I and class II molecules.
Affiliations:Center for Neurobiology and Vaccine Development, Ophthalmology Research, Cedars-Sinai Medical Center Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
Reference Type:Literature
PubMed ID:17499382
Journal:Virus Res
Journal Volume:128
Article Pages:71-80
Journal ISSN:0168-1702
Article Chemical List:Epitopes, T-Lymphocyte;Peptides;Protein Sorting Signals;Recombinant Proteins;UL53 protein, Human herpesvirus 1;Viral Proteins;Interferon-gamma
Article MeSH List:Amino Acid Sequence; Animals; CD4-Positive T-Lymphocytes(immunology); CD8-Positive T-Lymphocytes(immunology); Cells, Cultured; Epitope Mapping; Epitopes, T-Lymphocyte(immunology); Female; Herpesvirus 1, Human(chemistry; genetics; immunology); Interferon-gamma(biosynthesis); Lymphocyte Activation; Mice; Molecular Sequence Data; Peptides(chemical synthesis; chemistry; immunology); Protein Sorting Signals(genetics); Rabbits; Recombinant Proteins; Signal Transduction; Spodoptera; T-Lymphocytes(immunology); Viral Proteins(chemistry; genetics; immunology)
Curation Last Updated:2015-07-30 20:18:08