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| Article Authors: | Carla Lo Passo; Angela Romeo; Ida Pernice; Paolo Donato; Angelina Midiri; Giuseppe Mancuso; Milena Arigò; Carmelo Biondo; Roberta Galbo; Salvatore Papasergi; Franco Felici; Giuseppe Teti; Concetta Beninati |
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| Article Title: | Peptide mimics of the group B meningococcal capsule induce bactericidal and protective antibodies after immunization. |
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| Reference ID: | 1006162 |
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| Abstract: | Neisseria meningitidis serogroup B (MenB) is a leading cause of sepsis and meningitis in children. No vaccine is available for the prevention of these infections because the group B capsular polysaccharide (CP) (MenB CP) is unable to stimulate an immune response, due to its similarity with human polysialic acid. Because the MenB CP bears both human cross-reactive and non-cross-reactive determinants, we developed immunogenic peptide mimics of the latter epitopes. Peptides were selected from phage display libraries for their ability to bind to a protective anti-MenB CP mAb. One of these peptides (designated 9M) induced marked elevations in serum bactericidal activity, but not polysialic acid cross-reacting Abs, after gene priming followed by carrier-conjugate boosting. Moreover, the occurrence of bacteremia was prevented in infant rats by administration of immune sera before MenB challenge. 9M is a promising lead candidate for the development of an effective and affordable anti-MenB vaccine. |
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| Affiliations: | Dipartimento di Scienze Microbiologiche, Genetiche e Molecolari, Università degli Studi di Messina, Messina, Italy. |
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| Date: | 2007 |
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| Reference Type: | Literature |
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| PubMed ID: | 17371999 |
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| Journal: | J Immunol |
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| Journal Volume: | 178 |
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| Article Pages: | 4417-23 |
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| Journal ISSN: | 0022-1767 |
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| Article Chemical List: | Antibodies, Bacterial;Antibodies, Monoclonal;Bacterial Capsules;Immune Sera;Meningococcal Vaccines;Peptide Library;Peptides;Polysaccharides, Bacterial;capsular polysaccharide, meningococcal group B;peptide 9M;DNA |
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| Article MeSH List: | Amino Acid Sequence; Animals; Antibodies, Bacterial(blood); Antibodies, Monoclonal(immunology); Bacteremia(prevention & control); Bacterial Capsules; DNA(genetics); Immune Sera(immunology); Immunization; Immunization, Passive; Meningococcal Infections(prevention & control); Meningococcal Vaccines(immunology); Mice; Mice, Inbred BALB C; Molecular Mimicry; Molecular Sequence Data; Neisseria meningitidis, Serogroup B; Peptide Library; Peptides(genetics; immunology; pharmacology); Plasmids(genetics); Polysaccharides, Bacterial(chemistry; immunology); Rats; Rats, Inbred BB |
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| Curation Last Updated: | 2013-05-11 20:02:38 |
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