Epitopes described in "Peptide mimics of the group B meningococcal capsule induce bactericidal and protective antibodies after immunization."

Article Authors:Carla Lo Passo; Angela Romeo; Ida Pernice; Paolo Donato; Angelina Midiri; Giuseppe Mancuso; Milena Arigò; Carmelo Biondo; Roberta Galbo; Salvatore Papasergi; Franco Felici; Giuseppe Teti; Concetta Beninati
Article Title:Peptide mimics of the group B meningococcal capsule induce bactericidal and protective antibodies after immunization.
Reference Detail
Reference ID:1006162
Abstract:Neisseria meningitidis serogroup B (MenB) is a leading cause of sepsis and meningitis in children. No vaccine is available for the prevention of these infections because the group B capsular polysaccharide (CP) (MenB CP) is unable to stimulate an immune response, due to its similarity with human polysialic acid. Because the MenB CP bears both human cross-reactive and non-cross-reactive determinants, we developed immunogenic peptide mimics of the latter epitopes. Peptides were selected from phage display libraries for their ability to bind to a protective anti-MenB CP mAb. One of these peptides (designated 9M) induced marked elevations in serum bactericidal activity, but not polysialic acid cross-reacting Abs, after gene priming followed by carrier-conjugate boosting. Moreover, the occurrence of bacteremia was prevented in infant rats by administration of immune sera before MenB challenge. 9M is a promising lead candidate for the development of an effective and affordable anti-MenB vaccine.
Affiliations:Dipartimento di Scienze Microbiologiche, Genetiche e Molecolari, Università degli Studi di Messina, Messina, Italy.
Reference Type:Literature
PubMed ID:17371999
Journal:J Immunol
Journal Volume:178
Article Pages:4417-23
Journal ISSN:0022-1767
Article Chemical List:Antibodies, Bacterial;Antibodies, Monoclonal;Immune Sera;Meningococcal Vaccines;Peptide Library;Peptides;Polysaccharides, Bacterial;capsular polysaccharide, meningococcal group B;peptide 9M;DNA
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Bacterial(blood); Antibodies, Monoclonal(immunology); Bacteremia(prevention & control); Bacterial Capsules; DNA(genetics); Immune Sera(immunology); Immunization; Immunization, Passive; Meningococcal Infections(prevention & control); Meningococcal Vaccines(immunology); Mice; Mice, Inbred BALB C; Molecular Mimicry; Molecular Sequence Data; Neisseria meningitidis, Serogroup B; Peptide Library; Peptides(genetics; immunology; pharmacology); Plasmids(genetics); Polysaccharides, Bacterial(chemistry; immunology); Rats; Rats, Inbred BB
Curation Last Updated:2016-05-11 20:03:07