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| Article Authors: | Jia Zhu; David M Koelle; Jianhong Cao; Julio Vazquez; Meei Li Huang; Florian Hladik; Anna Wald; Lawrence Corey |
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| Article Title: | Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation. |
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| Reference ID: | 1004494 |
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| Abstract: | Cytotoxic CD8(+) T cells play a critical role in controlling herpes simplex virus (HSV) infection and reactivation. However, little is known about the spatiotemporal dynamics of CD8(+) T cells during HSV lesion evolution or about their involvement in immune surveillance after lesion resolution. Using quantum dot-conjugated peptide-major histocompatibility complex multimers, we investigated the in vivo localization of HSV-2-specific CD8(+) T cells in sequential biopsies of human genital skin during acute, resolving, and healed stages of HSV-2 reactivation. Our studies revealed that functionally active CD8(+) T cells selectively infiltrated to the site of viral reactivation. After lesion healing in concert with complete reepithelialization and loss of HSV DNA from skin biopsies, HSV-2-specific CD8(+) T cells persisted for more than two months at the dermal-epidermal junction, adjacent to peripheral nerve endings. In two out of the six sequentially studied individuals, HSV-2 DNA reappeared in clinically and histologically normal-appearing skin. Detection of viral DNA was accompanied by increased numbers of both HSV-specific and total CD8(+) T cells in the dermis. These findings indicate that the frequency and clinical course of HSV-2 reactivation in humans is influenced by virus-specific CD8(+) T cells that persist in peripheral mucosa and genital skin after resolution of herpes lesions. |
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| Affiliations: | Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA. |
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| Date: | 2007 |
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| Reference Type: | Literature |
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| PubMed ID: | 17325200 |
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| Journal: | J Exp Med |
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| Journal Volume: | 204 |
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| Article Pages: | 595-603 |
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| Journal ISSN: | 1540-9538 |
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| Article MeSH List: | Adult; CD8-Positive T-Lymphocytes(immunology; pathology; virology); Cell Movement(immunology); Female; Genitalia, Female(innervation); Genitalia, Male(innervation); Herpes Genitalis(immunology; pathology; virology); Herpesvirus 2, Human(immunology); Humans; Male; Middle Aged; Nerve Endings(immunology; pathology; virology); Skin Diseases, Viral(immunology; pathology; virology); Virus Activation(immunology) |
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| Curation Last Updated: | 2012-09-25 20:12:26 |
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