Epitopes described in "Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation."

Article Authors:Jia Zhu; David M Koelle; Jianhong Cao; Julio Vazquez; Meei Li Huang; Florian Hladik; Anna Wald; Lawrence Corey
Article Title:Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation.
Reference Detail
Reference ID:1004494
Abstract:Cytotoxic CD8(+) T cells play a critical role in controlling herpes simplex virus (HSV) infection and reactivation. However, little is known about the spatiotemporal dynamics of CD8(+) T cells during HSV lesion evolution or about their involvement in immune surveillance after lesion resolution. Using quantum dot-conjugated peptide-major histocompatibility complex multimers, we investigated the in vivo localization of HSV-2-specific CD8(+) T cells in sequential biopsies of human genital skin during acute, resolving, and healed stages of HSV-2 reactivation. Our studies revealed that functionally active CD8(+) T cells selectively infiltrated to the site of viral reactivation. After lesion healing in concert with complete reepithelialization and loss of HSV DNA from skin biopsies, HSV-2-specific CD8(+) T cells persisted for more than two months at the dermal-epidermal junction, adjacent to peripheral nerve endings. In two out of the six sequentially studied individuals, HSV-2 DNA reappeared in clinically and histologically normal-appearing skin. Detection of viral DNA was accompanied by increased numbers of both HSV-specific and total CD8(+) T cells in the dermis. These findings indicate that the frequency and clinical course of HSV-2 reactivation in humans is influenced by virus-specific CD8(+) T cells that persist in peripheral mucosa and genital skin after resolution of herpes lesions.
Affiliations:Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.
Reference Type:Literature
PubMed ID:17325200
Journal:J Exp Med
Journal Volume:204
Article Pages:595-603
Journal ISSN:0022-1007
Article MeSH List:Adult; CD8-Positive T-Lymphocytes(immunology; pathology; virology); Cell Movement(immunology); Female; Genitalia, Female(innervation); Genitalia, Male(innervation); Herpes Genitalis(immunology; pathology; virology); Herpesvirus 2, Human(immunology); Humans; Male; Middle Aged; Nerve Endings(immunology; pathology; virology); Skin Diseases, Viral(immunology; pathology; virology); Virus Activation(immunology)
Curation Last Updated:2015-01-17 21:36:03