Epitopes described in "Identification of the main epitope on human cytochrome P450 IID6 recognized by anti-liver kidney microsome antibody."

Article Authors:M Gueguen; O Boniface; O Bernard; F Clerc; T Cartwright; F Alvarez
Article Title:Identification of the main epitope on human cytochrome P450 IID6 recognized by anti-liver kidney microsome antibody.
Reference Detail
Reference ID:1004024
Abstract:Antibodies present in the sera of a group of children with autoimmune hepatitis react with human cytochrome P450 IID6. cDNA constructions of various fragments of human P450 IID6 were made and expressed and the resulting peptides were tested in immunoblot with patients' sera. These allowed identification of at least two antigenic sites on the P450 molecule. The main one, recognized by all sera tested, is located between amino acids 239 and 271. Synthesis of three peptides covering this area of the molecule allowed identification of a sequence of three amino acids (tyrosine-tryptophane-asparagine) located at position 261-263 that constitutes the essential part of the epitope. A protein sequence data-base search revealed homologies between this region of human P450 and proteins from Salmonella typhimurium, from human T lymphotropic virus types 1 and 2 and Herpes simplex virus type 1.
Affiliations:Institut National de la Santé et de la Recherche Médicale (INSERM U 56), Hôpital de Bicêtre, France.
Reference Type:Literature
PubMed ID:1723273
Journal:J Autoimmun
Journal Volume:4
Article Pages:607-15
Journal ISSN:0896-8411
Article Chemical List:Autoantibodies;Epitopes;Recombinant Fusion Proteins;anti-liver kidney microsome antibody;Cytochrome P-450 Enzyme System
Article MeSH List:Amino Acid Sequence; Animals; Autoantibodies(genetics ); Blotting, Western; Child; Child, Preschool; Cloning, Molecular; Cytochrome P-450 Enzyme System(genetics; immunology ); Epitopes(genetics; immunology ); Gene Expression; Hepatitis, Chronic(genetics; immunology ); Humans; Molecular Sequence Data; Plasmids; Rats; Recombinant Fusion Proteins; Sequence Alignment
Curation Last Updated:2014-10-03 20:31:14