Epitopes described in "Identification of monoclonal antibody epitopes and critical residues for rhinovirus binding in domain 1 of intercellular adhesion molecule 1."

Article Authors:A McClelland; J deBear; S C Yost; A M Meyer; C W Marlor; J M Greve
Article Title:Identification of monoclonal antibody epitopes and critical residues for rhinovirus binding in domain 1 of intercellular adhesion molecule 1.
Reference Detail
Reference ID:1009032
Abstract:Intercellular adhesion molecule 1 (ICAM-1) is the cellular receptor for the major group of human rhinoviruses (HRVs) and the adhesion ligand of lymphocyte function-associated antigen 1. Analysis of a series of chimeric exchanges between human and murine ICAM-1 shows that two distinct epitopes recognized by monoclonal antibodies that block rhinovirus attachment and cell adhesion map to the N-terminal first domain of ICAM-1. Furthermore the specificity for HRV binding is entirely contained within the first 88 amino acids. Mutagenesis of the four sites of N-linked glycosylation within the second domain shows that carbohydrate is not involved in virus recognition. Homologue replacement mutagenesis localizes the epitopes for virus-blocking antibodies to two regions of domain 1 predicted to form beta strand D and the loop between the F and G strands of an immunoglobulin-fold structure. Analysis of virus binding to the mutants predicts a large surface of contact between HRV and ICAM-1 domain 1 but shows that the regions most important for virus binding are coincident with the monoclonal antibody epitopes.
Affiliations:Molecular Therapeutics, Inc., Miles Research Center, West Haven, CT 06516.
Reference Type:Literature
PubMed ID:1716769
Journal:Proc Natl Acad Sci U S A
Journal Volume:88
Article Pages:7993-7
Journal ISSN:1091-6490
Article Chemical List:Antibodies, Monoclonal;Antibodies, Viral;Antigens, Viral;Cell Adhesion Molecules;Epitopes;Receptors, Virus;Recombinant Fusion Proteins;Intercellular Adhesion Molecule-1
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal(immunology); Antibodies, Viral(immunology); Antigens, Viral(immunology); Cell Adhesion Molecules(chemistry; immunology; metabolism); Computer Graphics; DNA Mutational Analysis; Epitopes; Humans; Intercellular Adhesion Molecule-1; Mice; Models, Molecular; Molecular Sequence Data; Receptors, Virus(immunology; metabolism); Recombinant Fusion Proteins; Rhinovirus(immunology); Structure-Activity Relationship
Curation Last Updated:2014-10-03 21:03:30