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| Article Authors: | Elizabeth Alice Louise Bateman; Michael Roger Ardern-Jones; Graham Stuart Ogg |
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| Article Title: | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
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| Reference ID: | 1005053 |
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| Abstract: | BACKGROUND: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. OBJECTIVE: To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. METHODS: Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. CONCLUSION: Circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. CLINICAL IMPLICATIONS: Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease. |
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| Affiliations: | Medical Research Council Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom. |
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| Date: | 2006 |
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| Reference Type: | Literature |
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| PubMed ID: | 17137868 |
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| Journal: | J Allergy Clin Immunol |
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| Journal Volume: | 118 |
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| Article Pages: | 1350-6 |
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| Journal ISSN: | 0091-6749 |
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| Article Chemical List: | Allergens;Antigens, CD27;Antigens, CD28;CCR7 protein, human;Cytokines;Epitopes;Fel d 1 protein, Felis domesticus;Glycoproteins;HLA-DR Antigens;Receptors, CCR7;Receptors, Chemokine;L-Selectin;HLA-DRB1 antigen |
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| Article MeSH List: | Adult; Allergens(immunology); Antigens, CD27(metabolism); Antigens, CD28(metabolism); CD4-Positive T-Lymphocytes(immunology; metabolism); Cells, Cultured; Chronic Disease; Cytokines(metabolism); Dermatitis, Atopic(immunology); Epitopes(immunology); Glycoproteins(immunology); HLA-DR Antigens(immunology; metabolism); Humans; Immunologic Memory; L-Selectin(metabolism); Receptors, CCR7; Receptors, Chemokine(metabolism) |
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| Curation Last Updated: | 2012-01-25 20:01:07 |
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