Epitopes described in "Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells."

Article Authors:Elizabeth Alice Louise Bateman; Michael Roger Ardern-Jones; Graham Stuart Ogg
Article Title:Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells.
Reference Detail
Reference ID:1005053
Abstract:BACKGROUND: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. OBJECTIVE: To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. METHODS: Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. CONCLUSION: Circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. CLINICAL IMPLICATIONS: Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease.
Reference Type:Literature
PubMed ID:17137868
Journal:J Allergy Clin Immunol
Journal Volume:118
Article Pages:1350-6
Journal ISSN:0091-6749
Article Chemical List:Allergens;Antigens, CD27;Antigens, CD28;CCR7 protein, human;Cytokines;Epitopes;Fel d 1 protein, Felis domesticus;Glycoproteins;HLA-DR Antigens;HLA-DRB1 Chains;Receptors, CCR7;Receptors, Chemokine;L-Selectin
Article MeSH List:Adult; Allergens(immunology); Antigens, CD27(metabolism); Antigens, CD28(metabolism); CD4-Positive T-Lymphocytes(immunology; metabolism); Cells, Cultured; Chronic Disease; Cytokines(metabolism); Dermatitis, Atopic(immunology); Epitopes(immunology); Glycoproteins(immunology); HLA-DR Antigens(immunology; metabolism); HLA-DRB1 Chains; Humans; Immunologic Memory; L-Selectin(metabolism); Receptors, CCR7; Receptors, Chemokine(metabolism)
Curation Last Updated:2015-01-27 19:19:36