Epitopes described in "Peptide selection by MHC class I molecules."

Article Authors:T N Schumacher; M L De Bruijn; L N Vernie; W M Kast; C J Melief; J J Neefjes; H L Ploegh
Article Title:Peptide selection by MHC class I molecules.
Reference Detail
Reference ID:1013130
Abstract:Synthetic peptides have been used to sensitize target cells and thereby screen for epitopes recognized by T cells. Most epitopes of cytotoxic T lymphocytes can be mimicked by synthetic peptides of 12-15 amino acids. Although in specific cases, truncations of peptides improves sensitization of target cells, no optimum length for binding to major histocompatibility complex (MHC) class I molecules has been defined. We have now analysed synthetic peptide captured by empty MHC class I molecules of the mutant cell line RMA-S. We found that class I molecules preferentially bound short peptides (nine amino acids) and selectively bound these peptides even when they were a minor component in a mixture of longer peptides. These results may help to explain the difference in size restriction of T-cell epitopes between experiments with synthetic peptides and those with naturally processed peptides.
Affiliations:Department of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam.
Reference Type:Literature
PubMed ID:1708852
Journal Volume:350
Article Pages:703-6
Journal ISSN:0028-0836
Article Chemical List:Epitopes;Histocompatibility Antigens Class I;Oligopeptides
Article MeSH List:Amino Acid Sequence; Animals; Cell Line; Cell Transformation, Neoplastic; Epitopes(analysis; immunology ); Histocompatibility Antigens Class I(immunology ); Mice; Molecular Sequence Data; Oligopeptides(chemical synthesis; immunology ); Protein Binding; Rauscher Virus(genetics ); T-Lymphocytes, Cytotoxic(immunology )
Curation Last Updated:2015-07-30 20:25:09