Epitopes described in "Anti-cyclic citrullinated peptide positivity in non-rheumatoid arthritis disease samples: citrulline-dependent or not?"

Article Authors:A Vannini; K Cheung; M Fusconi; J Stammen-Vogelzangs; J P H Drenth; A C Dall'Aglio; F B Bianchi; L E Bakker-Jonges; W J van Venrooij; G J M Pruijn; A J W Zendman
Article Title:Anti-cyclic citrullinated peptide positivity in non-rheumatoid arthritis disease samples: citrulline-dependent or not?
Reference Detail
Reference ID:1006932
Abstract:BACKGROUND: Antibodies directed against citrullinated proteins (eg anti-cyclic citrullinated peptide (CCP)) have excellent diagnostic and good prognostic potential for rheumatoid arthritis. Type 1 autoimmune hepatitis (AIH-1) is a chronic liver disease characterised by a variety of serum autoantibodies. Recently, in a large group of patients with AIH-1 without clear rheumatoid arthritis overlap, a relatively high percentage (9%) of anti-CCP2 positivity was scored. OBJECTIVES: To characterise the citrulline-dependence of the observed anti-CCP2 positivity in AIH-1 sera as well as in other groups of patients without rheumatoid arthritis (mainly rheumatic diseases). METHODS: Serum samples of 57 patients with AIH-1 and 66 patients without rheumatoid arthritis, most of them reported as anti-CCP positive, were tested for citrulline-specific reactivity with a second generation anti-CCP kit, with the citrullinated and the corresponding non-citrullinated (arginine-containing) antigen. A subset of AIH-1 sera was also tested with a CCP1 ELISA (and arginine control). RESULTS: The anti-CCP2 reactivity of most non-rheumatoid arthritis rheumatic diseases samples (87-93%) was citrulline-specific, whereas a relatively high percentage of AIH-1 samples (42-50%) turned out to be reactive in a citrulline-independent manner. The use of citrullinated and non-citrullinated CCP1 peptides confirmed a high occurrence of citrulline-independent reactivity in AIH-1 samples. CONCLUSIONS: In rheumatoid arthritis and most non-rheumatoid arthritis rheumatologic disease sera, anti-CCP positivity is citrulline-dependent. However in some patients, particularly patients with AIH-1, citrulline-independent reactivity in the anti-CCP2 test can occur. A positive CCP test in a non-rheumatic disease (eg liver disease) should therefore be interpreted with care, and preferably followed by a control ELISA with a non-citrullinated antigen.
Affiliations:Department of Internal Medicine, Cardioangiology, Hepatology, University of Bologna, and Azienda Ospedaliero-Universitaria S. Orsola-Malpighi, Bologna, Italy. antoniovannini@tiscali.it.
Reference Type:Literature
PubMed ID:16984940
Journal:Ann Rheum Dis
Journal Volume:66
Article Pages:511-6
Journal ISSN:0003-4967
Article Chemical List:Autoantibodies;Autoantigens;Biological Markers;Epitopes;Peptides, Cyclic;Reagent Kits, Diagnostic;cyclic citrullinated peptide;Citrulline
Article MeSH List:Adolescent; Adult; Aged; Aged, 80 and over; Arthritis(diagnosis; immunology); Arthritis, Rheumatoid(diagnosis; immunology); Autoantibodies(blood); Autoantigens(immunology); Biological Markers(blood); Child; Citrulline(immunology); Enzyme-Linked Immunosorbent Assay(methods); Epitopes; Female; Hepatitis, Autoimmune(immunology); Humans; Male; Middle Aged; Peptides, Cyclic(immunology); Reagent Kits, Diagnostic; Rheumatic Diseases(immunology)
Curation Last Updated:2015-12-28 20:01:53