Epitopes described in "Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins."

Article Authors:Carrie A Batten; Ian N Clarke; Sarah L Kempster; Stefan L Oliver; Janice C Bridger; Paul R Lambden
Article Title:Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins.
Reference Detail
Reference ID:1007986
Abstract:The Southampton norovirus (SV) capsid protein was expressed as VLPs by recombinant baculoviruses in insect cells and was used to immunize mice for the production of monoclonal antibodies (mAbs). One mAb, CM54, showed broad cross-reactivity to genogroup I (GI) noroviruses, but was not reactive to GII capsid proteins. Interestingly mAb CM54 reacted to a bovine norovirus capsid protein. Immunoblot analysis indicated the binding site for CM54 was located in the shell domain between amino acid residues 102-225 of the SV capsid protein. The epitope was mapped to high resolution using a peptide array and was located to the sequence LEDVRN at amino acid residues 162-167. Alignment of norovirus capsid protein sequences confirmed the epitope sequence was common to particular groups of human and bovine noroviruses. Modeling of the epitope onto the recombinant NV capsid protein revealed it was located to the inner surface of the shell domain.
Affiliations:Molecular Microbiology Group, University Medical School, Southampton General Hospital, Southampton SO16 6YD, UK. carrie.batten@bbsrc.ac.uk
Reference Type:Literature
PubMed ID:16934306
Journal Volume:356
Article Pages:179-87
Journal ISSN:0042-6822
Article Chemical List:Antibodies, Monoclonal;Antibodies, Viral;Capsid Proteins;Epitopes;Norwalk virus capsid
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal(biosynthesis; immunology ); Antibodies, Viral(biosynthesis; immunology ); Baculoviridae(genetics; metabolism ); Capsid Proteins(genetics; immunology ); Cattle; Cells, Cultured; Cross Reactions; Epitope Mapping; Epitopes; Genotype; Humans; Immunization; Models, Molecular; Molecular Sequence Data; Norovirus(immunology ); Recombination, Genetic; Spodoptera
Curation Last Updated:2014-10-03 20:59:46