Epitopes described in "Subtle differences in HLA DQ haplotype-associated presentation of AChR alpha-chain peptides may suffice to mediate myasthenia gravis."

Article Authors:Philip R Deitiker; Minako Oshima; R Glenn Smith; Dennis R Mosier; M Zouhair Atassi
Article Title:Subtle differences in HLA DQ haplotype-associated presentation of AChR alpha-chain peptides may suffice to mediate myasthenia gravis.
Reference Detail
Reference ID:1016879
Abstract:The HLA DQA1 and DQB1 alleles were determined on a set of 24 myasthenia gravis patients that had previously been examined for their T-cell proliferative responses to the 18 overlapping peptides representing the extracellular domain of hAChR alpha-chain. Patient responses according to assumed cis or trans haplotypes were significantly higher in most cases relative to normal controls. Comparisons of in vitro peptide-stimulated T-cell responses of patient pairs which had DQA1:DQB1 in common displayed responses in tighter distribution relative to comparisons in which patient pairs did not share the same DQA1:DQB1 haplotype. Similar haplotypes, such as DQA1*0102:DQB1*0602 and DQA1*0102:DQB1*0604, tended to exhibit similar responses and were grouped according to this similarity. Modified F-test and Student's T-test analyses on DQ isoform bearing groups revealed that high responses to peptide alpha34-49 were associated with A1*0102:B1*0602/0604, A1*0301:B1*0302 and A1*0401/0303:B1*0301. Peptide alpha146-162 showed higher responses in A1*0301:B1*0302 group and moderate responses in A1*0401/0303:B1*0301 groups. Differences in the age of disease onset relative to DQ haplotypes were also observed. Groups of A1*0301:B1*0302, A1*0501:B1*0201 and A1*0102:B1*0604 showed earlier ages of disease onset relative to those of A1*0102:B1*0602 or A1*0505:B1*0301.
Affiliations:Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Reference Type:Literature
PubMed ID:16891216
Journal Volume:39
Article Pages:277-88
Journal ISSN:0891-6934
Article Chemical List:HLA-DQ Antigens;HLA-DQ alpha-Chains;HLA-DQ beta-Chains;HLA-DQA1 antigen;HLA-DQB1 antigen;Membrane Glycoproteins;Protein Subunits;Receptors, Cholinergic
Article MeSH List:Adolescent; Adult; Age Factors; Aged; Alleles; Amino Acid Sequence; Female; Genotype; HLA-DQ Antigens(genetics; immunology); HLA-DQ alpha-Chains; HLA-DQ beta-Chains; Haplotypes; Humans; Lymphocyte Activation; Male; Membrane Glycoproteins(genetics; immunology); Middle Aged; Molecular Sequence Data; Myasthenia Gravis(genetics; immunology); Protein Subunits(immunology); Receptors, Cholinergic(immunology); T-Lymphocytes(immunology)
Curation Last Updated:2015-01-27 21:08:26