Epitopes described in "Intranasal vaccination with SfbI or M protein-derived peptides conjugated to diphtheria toxoid confers protective immunity against a lethal challenge with Streptococcus pyogenes."

Reference
Article Authors:Kai Schulze; Colleen Olive; Thomas Ebensen; Carlos A Guzmán
Article Title:Intranasal vaccination with SfbI or M protein-derived peptides conjugated to diphtheria toxoid confers protective immunity against a lethal challenge with Streptococcus pyogenes.
Reference Detail
Reference ID:1002088
Abstract:We investigated whether intranasal immunisation with diphtheria toxoid (DT) conjugated polypeptides encompassing T and B cell epitopes of the SfbI protein (FNBR) or a conformational-constrained B cell epitope of the M1 protein (J8) was able to confer protection against lethal mucosal challenge with a heterologous Streptococcus pyogenes strain. To this end, BALB/c mice were immunised with the conjugates. Strong antigen-specific antibody responses were observed in both serum and mucosal secretions. Vaccinated mice were challenged 10 days after the last boost by the intranasal route. Animals receiving FNBR-DT co-administered with either the cholera toxin B subunit (CTB) or the TLR 2/6 agonist MALP-2 were efficiently protected against the virulent S. pyogenes strain (90% and 70% survival, respectively), whereas those immunised with J8-DT plus either CTB or MALP-2 showed intermediate levels of protection (60% and 40%, respectively). The obtained results indicate that in our experimental animal model peptide-based conjugate vaccines represent a valid alternative to protect against streptococcal infection.
Affiliations:Department of Vaccinology, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. ksc@gbf.de
Date:2006
Reference Type:Literature
PubMed ID:16828529
Journal:Vaccine
Journal Volume:24
Article Pages:6088-95
Journal ISSN:0264-410X
Article Chemical List:Adjuvants, Immunologic;Antibodies, Bacterial;Bacterial Proteins;Diphtheria Toxoid;Toll-Like Receptor 2;Toll-Like Receptor 6;Vaccines, Subunit;Cholera Toxin
Article MeSH List:Adjuvants, Immunologic; Administration, Intranasal; Animals; Antibodies, Bacterial(analysis); Antibody Formation; Bacterial Proteins(immunology); Cholera Toxin; Diphtheria Toxoid; Female; Immunity, Cellular; Mice; Mice, Inbred BALB C; Mucus(chemistry); Serum(chemistry); Streptococcal Infections(immunology; prevention & control); Streptococcus pyogenes(immunology); Toll-Like Receptor 2(agonists); Toll-Like Receptor 6(agonists); Vaccines, Subunit(administration & dosage; therapeutic use)
Curation Last Updated:2013-05-28 20:32:24