Epitopes described in "Intranasal administration is an effective mucosal vaccine delivery route for self-adjuvanting lipid core peptides targeting the group A streptococcal M protein."

Article Authors:Colleen Olive; Hsien Kuo Sun; Mei-Fong Ho; Joanne Dyer; Aniko Horváth; Istvan Toth; Michael F Good
Article Title:Intranasal administration is an effective mucosal vaccine delivery route for self-adjuvanting lipid core peptides targeting the group A streptococcal M protein.
Reference Detail
Reference ID:1002089
Abstract:BACKGROUND: We investigated the lipid core peptide (LCP) system for mucosal vaccine delivery against infection with group A streptococcus (GAS)--the causative pathogen of rheumatic fever and rheumatic heart disease. METHODS: An LCP vaccine formulation containing 2 different peptide epitopes of the antiphagocytic M protein of GAS--a conformational epitope from the carboxyterminal conserved C-repeat region and an aminoterminal serotypic epitope--was intranasally administered to mice with cholera toxin B subunit or without additional adjuvant. RESULTS: Our data demonstrate that the LCP vaccine formulation induced the elicitation of antigen-specific systemic immunoglobulin G responses when administered with or without cholera toxin B subunit, whereas cholera toxin B subunit was required for the induction of antigen-specific mucosal immunoglobulin A responses. Immune serum samples from vaccinated mice were capable of opsonization of a homologous GAS strain, as well as opsonization of a heterologous GAS strain. Furthermore, mice were protected from GAS challenge following immunization with the LCP vaccine formulation, even in the absence of additional adjuvant. CONCLUSIONS: These data support the potential of the LCP system in the development of a self-adjuvanting, synthetic, peptide-based mucosal GAS vaccine for the prevention of diseases caused by GAS.
Affiliations:Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, Queensland 4029, Australia. colleenO@qimr.edu.au
Reference Type:Literature
PubMed ID:16826479
Journal:J Infect Dis
Journal Volume:194
Article Pages:316-24
Journal ISSN:1537-6613
Article Chemical List:Adjuvants, Immunologic;Antibodies, Bacterial;Antigens, Bacterial;Bacterial Outer Membrane Proteins;Carrier Proteins;Diphtheria Toxoid;Epitopes;Immunoglobulin A;Immunoglobulin G;Lipids;Peptide Fragments;Streptococcal Vaccines;Vaccines, Subunit;streptococcal M protein
Article MeSH List:Adjuvants, Immunologic(administration & dosage); Administration, Intranasal; Administration, Oral; Amino Acid Sequence; Animals; Antibodies, Bacterial(biosynthesis; blood); Antigens, Bacterial(immunology); Bacterial Outer Membrane Proteins(immunology); Carrier Proteins(immunology); Diphtheria Toxoid(administration & dosage; immunology); Drug Delivery Systems(methods); Epitopes(immunology); Immunity, Mucosal(immunology); Immunoglobulin A(blood; immunology); Immunoglobulin G(blood; immunology); Immunotherapy, Active(methods); Lipids(administration & dosage; chemistry; immunology); Mice; Molecular Sequence Data; Peptide Fragments(chemistry; immunology); Streptococcal Infections(immunology; prevention & control); Streptococcal Vaccines(administration & dosage; immunology); Streptococcus pyogenes(immunology); Vaccines, Subunit(administration & dosage; immunology)
Curation Last Updated:2016-01-09 20:03:42