Epitopes described in "Epitope mapping of full-length glycoprotein D from HSV-2 reveals a novel CD4+ CTL epitope located at the transmembrane-cytoplasmic junction."

Article Authors:David Cooper; Joseph C Mester; Min Guo; Farooq Nasar; Victor Souza; Sharon Dispoto; Maninder Sidhu; Michael Hagen; John H Eldridge; Robert J Natuk; Michael W Pride
Article Title:Epitope mapping of full-length glycoprotein D from HSV-2 reveals a novel CD4+ CTL epitope located at the transmembrane-cytoplasmic junction.
Reference Detail
Reference ID:1004525
Abstract:The glycoprotein D of HSV-2 (gD2) is currently a leading candidate vaccine target for genital herpes vaccines as both cellular and humoral responses can be generated against it. However, little is known about how vaccine composition will affect T cell epitope selection. A panel of 15-mer peptides (with 11 amino acid overlap) spanning full-length gD2 was used to investigate the fine specificity of T cell responses to gD2 as well as the role of vaccine composition on epitope selection. Spleen cells from BALB/c mice (H-2(d)) immunized with gD2, formulated with or without AlPO(4) and/or IL-12, were stimulated in vitro with overlapping gD2 peptides. Cellular responses (lymphoproliferation and IFN-gamma expression) were mapped to four epitopes within the gD2 molecule: gD2(49-63), gD2(105-119), gD2(245-259), and gD2(333-347). CTL analysis of these four epitopes indicated that not all of them could serve as a CTL epitope. Mice immunized with gD2 expressed from a viral vector mounted CTL responses primarily to one epitope located in the extracellular domain of gD2 (gD2(245-259)). More importantly, mice immunized with gD2 co-administered with IL-12 mounted CTL responses to an additional epitope located at the transmembrane-cytoplasmic junction of gD2 (gD2(333-347)). The location of this novel epitope emphasizes the benefit of using full-length versions of glycoproteins when designing vaccine components.
Affiliations:Department of Vaccines Discovery Research, Wyeth Research, Pearl River, NY 10965, USA. cooperd3@wyeth.com
Reference Type:Literature
PubMed ID:16762332
Journal:Cell Immunol
Journal Volume:239
Article Pages:113-20
Journal ISSN:0008-8749
Article Chemical List:Antigens, CD4;Epitopes, T-Lymphocyte;Peptide Fragments;Viral Envelope Proteins;glycoprotein D-herpes simplex virus type 2
Article MeSH List:Amino Acid Sequence; Animals; Antigens, CD4(metabolism ); CHO Cells; Cell Membrane(immunology ); Cercopithecus aethiops; Cricetinae; Cricetulus; Cytoplasm(immunology ); Epitope Mapping; Epitopes, T-Lymphocyte(biosynthesis; genetics ); Female; Herpesvirus 2, Human(genetics; immunology ); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptide Fragments(immunology ); T-Lymphocytes, Cytotoxic(immunology; metabolism ); Vero Cells; Viral Envelope Proteins(genetics; immunology )
Curation Last Updated:2015-07-30 20:16:06